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验证数据展示

  • WB analysis using 33335-1-AP

    WB analysis using 33335-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 33335-1-AP (MAST2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 33335-1-AP

    IF Staining of A431 using 33335-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using MAST2 antibody (33335-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of U2OS using 33335-1-AP

    IF Staining of U2OS using 33335-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using MAST2 antibody (33335-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

MAST2 Polyclonal antibody

MAST2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 33335-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

EC:2.7.11.1, KIAA0807, MAST205, Microtubule-associated serine/threonine-protein kinase 2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, Jurkat cells
Positive IF/ICC detected inU2OS cells, A431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

33335-1-AP targets MAST2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag35818

Product name: Recombinant human MAST2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1480-1798 aa of NM_015112.2

Sequence: TLRQDRAERRESLQKQEAIREVDSSEDDTEEGPENSQGAQELSLAPHPEVSQSVAPKGAGESGEEDPFPSRDPRSLGPMVPSLLTGITLGPPRMESPSGPHRRLGSPQAIEEAASSSSAGPNLGQSGATDPIPPEGCWKAQHLHTQALTALSPSTSGLTPTSSCSPPSSTSGKLSMWSWKSLIEGPDRASPSRKATMAGGLANLQDLENTTPAQPKNLSPREQGKTQPPSAPRLAHPSYEDPSQGWLWESECAQAVKEDPALSITQVPDASGDRRQDVPCRGCPLTQKSEPSLRRGQEPGGHQKHRDLALVPDELLKQT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 microtubule associated serine/threonine kinase 2
别名 EC:2.7.11.1, KIAA0807, MAST205, Microtubule-associated serine/threonine-protein kinase 2
计算分子量196kDa，1798aa
观测分子量196 kDa
GenBank蛋白编号NM_015112.2
基因名称 MAST2
Gene ID (NCBI) 23139
RRIDAB_3742944
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ6P0Q8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAST2 is a microtubule-associated serine/threonine kinase, belonging to an evolutionarily conserved branch of AGC protein kinase superfamily. Since its discovery in 1993, it has been widely expressed in many tissues, including brain, heart, lung, liver, intestine and kidney. MAST2 is associated with many diseases, such as breast cancer, liver cancer, myeloma, inflammatory bowel disease, cystic fibrosis and various neurological diseases.

实验方案

Product Specific Protocols
IF protocol for MAST2 antibody 33335-1-APDownload protocol
WB protocol for MAST2 antibody 33335-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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