验证数据展示
经过测试的应用
| Positive WB detected in | SH-SY5Y cells, mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13305-1-AP targets MAST1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag4126 Product name: Recombinant human MAST1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 494-769 aa of BC027985 Sequence: TNDLVLRRARHQQMSGDVAVEKRPSRTGGKVIKSASATALSVMIPAVDPHGSSPLASPMSPRSLSSNPSSRDSSPSRDYSPAVSGLRSPITIQRSGKKYGFTLRAIRVYMGDTDVYSVHHIVWHVEEGGPAQEAGLCAGDLITHVNGEPVHGMVHPEVVELILKSGNKVAVTTTPFENTSIRIGPARRSSYKAKMARRNKRPSAKEGQESKKRSSLFRKITKQSNLLHTSRSLSSLNRSLSSSDSLPGSPTHGLPARSPTHSYRSTPDSAYLGASS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | microtubule associated serine/threonine kinase 1 |
| 别名 | SAST170, SAST, Microtubule-associated serine/threonine-protein kinase 1, MAST 1, KIAA0973 |
| 计算分子量 | 1570 aa, 171 kDa |
| 观测分子量 | 171 kDa |
| GenBank蛋白编号 | BC027985 |
| 基因名称 | MAST1 |
| Gene ID (NCBI) | 22983 |
| RRID | AB_10639043 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y2H9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MAST1(microtubule associated serine/threonine kinase 1) is also named as KIAA0973, SAST(syntrophin-associated serine/threonine-protein kinase), SAST170 and belongs to the family of microtubule-associated serine/threonine kinases, which has a unique structural feature with a serine/threonine kinase domain and a postsynaptic density protein-95/discs large/zona occludens-1 domain(PMID:12614677). This protein plays a role in a broad range of neural functions and it appears to link the dystrophin/utrophin network with microtubule filaments via the syntrophins.
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for MAST1 antibody 13305-1-AP | Download protocol |
| WB protocol for MAST1 antibody 13305-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Neuron Mutations in MAST1 Cause Mega-Corpus-Callosum Syndrome with Cerebellar Hypoplasia and Cortical Malformations.
| ||
Theranostics CRISPR/Cas9-based genome-wide screening for deubiquitinase subfamily identifies USP1 regulating MAST1-driven cisplatin-resistance in cancer cells |
