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验证数据展示

  • WB analysis using 67739-1-Ig

    WB analysis using 67739-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67739-1-Ig (MARS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of A431 using 67739-1-Ig

    WB analysis of A431 using 67739-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 67739-1-Ig (MARS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 67739-1-Ig

    IF Staining of HepG2 using 67739-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MARS antibody (67739-1-Ig, Clone: 3E3B4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • WB analysis of HepG2 using 67739-1-Ig

    WB analysis of HepG2 using 67739-1-Ig

    WB result of MARS antibody (67739-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MARS transfected HepG2 cells.

  • Featured Product
  • KD/KO验证

MARS Monoclonal antibody

MARS Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67739-1-Ig

产品说明书

CloneNo. 3E3B4

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

MARS1, 3E3B4, EC:6.1.1.10, Methionine--tRNA ligase, cytoplasmic, Methionyl-tRNA synthetase

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, A431 cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy MARS1 Ready-To-Use IHC Kit. MARS1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67739-1-Ig targets MARS in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag6961

Product name: Recombinant human MARS protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 552-900 aa of BC002384

Sequence: MAKDNVPFHSLVFPCSALGAEDNYTLVSHLIATEYLNYEDGKFSKSRGVGVFGDMAQDTGIPADIWRFYLLYIRPEGQDSAFSWTDLLLKNNSELLNNLGNFINRAGMFVSKFFGGYVPEMVLTPDDQRLLAHVTLELQHYHQLLEKVRIRDALRSILTISRHGNQYIQVNEPWKRIKGSEADRQRAGTVTGLAVNIAALLSVMLQPYMPTVSATIQAQLQLPPPACSILLTNFLCTLPAGHQIGTVSPLFQKLENDQIESLRQRFGGGQAKTSPKPAVVETVTTAKPQQIQALMDEVTKQGNIVRELKAQKADKNEVAAEVAKLLDLKKQLAVAEGKPPEAPKGKKKK

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 methionyl-tRNA synthetase
别名 MARS1, 3E3B4, EC:6.1.1.10, Methionine--tRNA ligase, cytoplasmic, Methionyl-tRNA synthetase
计算分子量 101 kDa
观测分子量 97-101 kDa
GenBank蛋白编号BC002384
基因名称 MARS
Gene ID (NCBI) 4141
RRIDAB_2918508
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP56192
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for MARS antibody 67739-1-IgDownload protocol
WB protocol for MARS antibody 67739-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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