- Featured Product
- KD/KO Validated
MARS Polyclonal antibody
MARS Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
IF/ICC, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy MARS1 Ready-To-Use IHC Kit. MARS1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 3 publications below |
| WB | See 4 publications below |
| IHC | See 1 publications below |
产品信息
14829-1-AP targets MARS in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.
| Tested Applications | IF/ICC, IHC, IP, WB, ELISA |
| Cited Applications | WB, IHC |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Immunogen | MARS fusion protein Ag6619 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | methionyl-tRNA synthetase |
| Synonyms | MARS, MARS1, Methionine tRNA ligase, methionyl tRNA synthetase, METRS, MTRNS |
| Calculated Molecular Weight | 101 kDa |
| Observed Molecular Weight | 101 kDa |
| GenBank Accession Number | BC002384 |
| Gene Symbol | MARS |
| Gene ID (NCBI) | 4141 |
| RRID | AB_2140917 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P56192 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MARS antibody 14829-1-AP | Download protocol |
| IHC protocol for MARS antibody 14829-1-AP | Download protocol |
| IF protocol for MARS antibody 14829-1-AP | Download protocol |
| IP protocol for MARS antibody 14829-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Proteomics Motile hepatocellular carcinoma cells preferentially secret sugar metabolism regulatory proteins via exosomes. | ||
J Clin Pathol High methionyl-tRNA synthetase expression predicts poor prognosis in patients with breast cancer.
| ||
Proc Natl Acad Sci U S A Multimodal cotranslational interactions direct assembly of the human multi-tRNA synthetase complex
| ||
Aging Cell N-homocysteinylation of α-synuclein promotes its aggregation and neurotoxicity
|
