MARCKSL1 Polyclonal antibody
MARCKSL1 Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
IHC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | human brain tissue |
| Positive IHC detected in | human lung cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
11422-1-AP targets MARCKSL1 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Applications | IHC, WB, ELISA |
| Cited Applications | WB |
| Tested Reactivity | human |
| Cited Reactivity | mouse |
| Immunogen | MARCKSL1 fusion protein Ag1967 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | MARCKS-like 1 |
| Synonyms | F52, Mac MARCKS, MACMARCKS, MARCKS like 1, MARCKS like protein 1, MARCKS related protein, MARCKSL1, MLP, MLP1, MRP |
| Calculated Molecular Weight | 195 aa, 20 kDa |
| Observed Molecular Weight | 42 kDa |
| GenBank Accession Number | BC007904 |
| Gene Symbol | MARCKSL1 |
| Gene ID (NCBI) | 65108 |
| RRID | AB_2140456 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P49006 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MARCKS-like protein 1 (MARCKSL1) is widely expressed in nervous tissue, it is also named MARCKS-like protein (MLP) or MARCKS-related protein (MRP) and belongs to the MARCKS family, which is a highly acidic myristoylated family of PKC substrates widely distributed in diverse cell types including macrophages. The protein has a calculated molecular weight of 20 kDa and an apparent molecular weight of 42-48 kDa due to anomalous migration on SDS gels or phosphorylation. Genetic disruption of MARCKSL1 results in neural tube closure defects, events that depend on coordinated control of actin functions, cell shape, and cell migration. MARCKSL1 are thought to regulate the actin cytoskeleton and thereby participate in major cellular responses such as phagocytosis, secretion, motility, mitogenesis, and membrane trafficking.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MARCKSL1 antibody 11422-1-AP | Download protocol |
| IHC protocol for MARCKSL1 antibody 11422-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
