验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
51087-1-AP targets MARCKS in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | myristoylated alanine-rich protein kinase C substrate |
| 别名 | 80K L, 80K L protein, MACS, MARCKS, PKCSL, PRKCSL |
| 计算分子量 | 32 kDa |
| 观测分子量 | 32 kDa, 80 kDa |
| GenBank蛋白编号 | BC089040 |
| 基因名称 | MARCKS |
| Gene ID (NCBI) | 4082 |
| RRID | AB_2881243 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P29966 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MARCKS(Myristoylated alanine-rich C-kinase substrate, 32 kDa ) is also named as MACS, PRKCSL and belongs to the MARCKS family. It is also named as protein kinase C substrate(80 kDa protein, light chain). MARCKS is a filamentous actincrosslinking protein that appears to function as an integrator of protein kinase C and calcium (Ca2+)/calmodulin signals in the regulation of actin-membrane interactions. This protein can be Phosphorylated by PKC displaces MARCKS from the membrane.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MARCKS antibody 51087-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
