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验证数据展示

  • WB analysis using 87790-1-RR

    WB analysis using 87790-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87790-1-RR (MARCH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse brain using 87790-1-RR

    IF Staining of mouse brain using 87790-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using MARCH5 antibody (87790-1-RR, Clone: 253335F11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MARCH5 Recombinant monoclonal antibody

MARCH5 Uni-rAb® Recombinant Antibody for WB, IF-P, ELISA
Cat No. 87790-1-RR

产品说明书

CloneNo. 253335F11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF-P, ELISA

别名

E3 ubiquitin-protein ligase MARCHF5, EC:2.3.2.27, MARCH V, MARCHF5, MARCH-V

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inU-937 cells, HuH-7 cells, mouse liver tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue, human skeletal muscle tissue
Positive IF-P detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87790-1-RR targets MARCH5 in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2806

Product name: Recombinant human MARCH5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-209 aa of BC015480

Sequence: MPDQALQQMLDRSCWVCFATDEDDRTAEWVRPCRCRGSTKWVHQACLQRWVDEKQRGNSTARVACPQCNAEYLIVFPKLGPVVYVLDLADRLISKACPFAAAGIMVGSIYWTAVTYGAVTVMQVVGHKEGLDVMERADPLFLLIGLPTIPVMLILGKMIRWEDYVLRLWRKYSNKLQILNSIFPGIGCPVPRIPAEANPLADHVSATRI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 membrane-associated ring finger (C3HC4) 5
别名 E3 ubiquitin-protein ligase MARCHF5, EC:2.3.2.27, MARCH V, MARCHF5, MARCH-V
计算分子量 278 aa, 31 kDa
观测分子量27-31 kDa
GenBank蛋白编号BC015480
基因名称 MARCH5
Gene ID (NCBI) 54708
RRIDAB_3745751
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9NX47
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MARCH5(E3 ubiquitin-protein ligase MARCH5) is a 31 kDa protein also named as RNF153. This protein plays a protective role against mitochondrial dysfunction caused by the mitochondrial accumulation of mSOD1 via the ubiquitin-proteasome pathway (PMID:19741096). And it may regulate MAP1B LC1 function in mitochondria by controlling mitochondrial aggregation via mitochondrial LC1 levels(PMID:22308378). MEARCH5 can exsit as a homodimer with MW 65-70 kDa.

实验方案

Product Specific Protocols
IF protocol for MARCH5 antibody 87790-1-RRDownload protocol
WB protocol for MARCH5 antibody 87790-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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