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验证数据展示

  • WB analysis of K-562 using 10364-1-AP

    WB analysis of K-562 using 10364-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 10364-1-AP (MAPRE2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse cells using 10364-1-AP

    IF Staining of mouse cells using 10364-1-AP

    Mouse keratinocyte showing MAPRE2 (#10364-1-AP, 1:100 dilution, with Alexa 594 labelled 2nd Ab) in red and alpha-tubulin (#66031-1-lg, 1:100 dilution, with Alexa 488 labelled 2nd Ab ) in green.

  • IHC staining of human stomach using 10364-1-AP

    IHC staining of human stomach using 10364-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10364-1-AP (MAPRE2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MAPRE2 antibody included
  • IF Staining of HCT 116 using 10364-1-AP

    IF Staining of HCT 116 using 10364-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HCT 116 cells using MAPRE2 antibody (10364-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Featured Product
  • KD/KO验证

MAPRE2 Polyclonal antibody

MAPRE2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 10364-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

APC binding protein EB2, APC-binding protein EB2, EB2, End-binding protein 2, Microtubule-associated protein RP/EB family member 2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inK-562 cells, Jurkat cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHCT 116 cells, mouse cells
Planning an IHC experiment? We recommend our IHCeasy MAPRE2 Ready-To-Use IHC Kit. MAPRE2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10364-1-AP targets MAPRE2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性mouse
免疫原

CatNo: Ag0416

Product name: Recombinant human MAPRE2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 6-327 aa of BC007318

Sequence: QTLSPNGENNNDIIQDNNGTIIPFRKHTVRGERSYSWGMAVNVYSTSITQETMSRHDIIAWVNDIVSLNYTKVEQLCSGAAYCQFMDMLFPGCISLKKVKFQAKLEHEYIHNFKLLQASFKRMNVDKVIPVEKLVKGRFQDNLDFIQWFKKFYDANYDGKEYDPVEARQGQDAIPPPDPGEQIFNLPKKSHHANSPTAGAAKSSPAAKPGSTPSRPSSAKRASSSGSASKSDKDLETQVIQLNEQVHSLKLALEGVEKERDFYFGKLREIELLCQEHGQENDDLVQRLMDILYASEEHEGHTEEPEAEEQAHEQQPPQQEEY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 microtubule-associated protein, RP/EB family, member 2
别名 APC binding protein EB2, APC-binding protein EB2, EB2, End-binding protein 2, Microtubule-associated protein RP/EB family member 2
计算分子量 37 kDa
观测分子量 39-42 kDa
GenBank蛋白编号BC007318
基因名称 MAPRE2
Gene ID (NCBI) 10982
RRIDAB_2141649
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15555
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RP1 protein shares significant homology to the adenomatous polyposis coli (APC) protein-binding EB1 gene family and binds specifically to the C-terminal region of the APC protein. RP1 also binds directly to tubulin, both in vitro and in vivo. The function of RP1 is currently unknown; however, its homology suggests involvement in tumorigenesis of colorectal cancers and proliferative control of normal cells. It may belong to the intermediate/early gene family, involved in the signal transduction cascade downstream of the TCR.

实验方案

Product Specific Protocols
IF protocol for MAPRE2 antibody 10364-1-APDownload protocol
IHC protocol for MAPRE2 antibody 10364-1-APDownload protocol
WB protocol for MAPRE2 antibody 10364-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Sci Adv

Global ubiquitylation analysis of mitochondria in primary neurons identifies endogenous Parkin targets following activation of PINK1.

Authors - Odetta Antico
View Article
mouseWB

Exp Cell Res

MAPRE2 regulates the first meiotic progression in mouse oocytes.

Authors - Yuan-Yuan Li
  • KD Validated
View Article
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