- Featured Product
- KD/KO Validated
MAPKAPK5 Polyclonal antibody
MAPKAPK5 Polyclonal Antibody for IF/ICC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, WB, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, Jurkat cells |
| Positive IF detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14339-1-AP targets MAPKAPK5 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC, WB, ELISA |
| Cited Applications | WB, IP, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | MAPKAPK5 fusion protein Ag5650 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | mitogen-activated protein kinase-activated protein kinase 5 |
| Synonyms | MAPKAP kinase 5, MAPKAPK 5, MAPKAPK5, PRAK |
| Calculated Molecular Weight | 54 kDa |
| Observed Molecular Weight | 54 kDa |
| GenBank Accession Number | BC047284 |
| Gene Symbol | MAPKAPK5 |
| Gene ID (NCBI) | 8550 |
| RRID | AB_2141473 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IW41 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MAPKAPK5(MAP kinase-activated protein kinase 5) is also named as PRAK and belongs to the CAMK Ser/Thr protein kinase family. MAPKAPK5 contains the conserved protein kinase domains I through XI, which are characteristic of all protein kinases. It is a major stress-activated kinase that can phosphorylate small heat shock protein, suggesting a potential role for PRAK in mediating stress-induced HSP27 phosphorylation in vivo(PMID:9628874). It has 2 isoforms produced by alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MAPKAPK5 antibody 14339-1-AP | Download protocol |
| IF protocol for MAPKAPK5 antibody 14339-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun The essential role of PRAK in tumor metastasis and its therapeutic potential.
| ||
J Cancer Biol Res Cross-Phosphorylation and Interaction between Src/FAK and MAPKAP5/PRAK in Early Focal Adhesions Controls Cell Motility. |
