SIN1 Polyclonal antibody
SIN1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | mouse kidney tissue, HeLa cells |
Positive IP detected in | mouse kidney tissue |
Positive IHC detected in | mouse kidney tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 4 publications below |
IF | See 1 publications below |
产品信息
15463-1-AP targets SIN1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF, IHC, IP, WB, ELISA |
Cited Applications | WB, IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Immunogen | SIN1 fusion protein Ag7753 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | mitogen-activated protein kinase associated protein 1 |
Synonyms | JC310, MAPKAP1, MIP1, mSIN1, SAPK interacting protein 1, SIN1, SIN1b, SIN1g, TORC2 subunit MAPKAP1 |
Calculated Molecular Weight | 59 kDa |
Observed Molecular Weight | 65 kDa |
GenBank Accession Number | BC002326 |
Gene Symbol | MAPKAP1 |
Gene ID (NCBI) | 79109 |
RRID | AB_10598466 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9BPZ7 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
---|---|
WB protocol for SIN1 antibody 15463-1-AP | Download protocol |
IHC protocol for SIN1 antibody 15463-1-AP | Download protocol |
IF protocol for SIN1 antibody 15463-1-AP | Download protocol |
IP protocol for SIN1 antibody 15463-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Rep Mitochondrial dynamics define muscle fiber type by modulating cellular metabolic pathways | ||
bioRxiv Host-derived oxidized phospholipids initiate effector-triggered immunity fostering lethality upon microbial encounter | ||
Nat Biotechnol Quantitative sequencing using BID-seq uncovers abundant pseudouridines in mammalian mRNA at base resolution | ||