验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, A549 cells, COS-7 cells, NIH/3T3 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 10 publications below |
| IHC | See 2 publications below |
产品信息
51153-1-AP targets JNK in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat, monkey |
| 文献引用反应性 | human, mouse, rat, rabbit |
| 免疫原 | JNK fusion protein Ag1442 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | mitogen-activated protein kinase 9 |
| 别名 | MAPK9, JNK2A, JNK2, JNK 55, EC:2.7.11.24 |
| 计算分子量 | 424 aa, 48 kDa |
| 观测分子量 | 44-55 kDa |
| GenBank蛋白编号 | BC032539 |
| 基因名称 | JNK |
| Gene ID (NCBI) | 5601 |
| RRID | AB_10898168 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P45984 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
c-Jun N-terminal kinases (JNKs), first characterized as stress-activated members of the mitogen-activated protein kinase (MAPK) family, have shown their critical roles in the development of a number of cellular progress and diseases, including proliferation, embryonic development, apoptosis, diabetes, neurodegeneration and liver disease (PMID: 19900593). JNK1, JNK2, JNK3 also name as MAPK8, MAPK9, MAPK10, JNK1 and JNK2 are ubiquitously expressed, but JNK3 is expressed primarily in the brain (PMID: 27698029). This antibody can recognize JNK1, JNK2 and JNK3, we get bands around 44-55 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for JNK antibody 51153-1-AP | Download protocol |
| IHC protocol for JNK antibody 51153-1-AP | Download protocol |
| IF protocol for JNK antibody 51153-1-AP | Download protocol |
| IP protocol for JNK antibody 51153-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Adv Mater m6 A Reader YTHDF1-Targeting Engineered Small Extracellular Vesicles for Gastric Cancer Therapy via Epigenetic and Immune Regulation | ||
Cell Rep Med Development of an orally bioavailable CDK12/13 degrader and induction of synthetic lethality with AKT pathway inhibition | ||
Nat Commun SH3RF3 promotes breast cancer stem-like properties via JNK activation and PTX3 upregulation. | ||
Biomed Pharmacother Icariside II suppresses cervical cancer cell migration through JNK modulated matrix metalloproteinase-2/9 inhibition in vitro and in vivo. | ||
Neurochem Res Exogenous TIPE2 Inhibit TAK1 to Improve Inflammation and Neuropathic Pain Induced by Sciatic Nerve Injury Through Inactivating NF-κB and JNK. | ||
Microb Pathog Polysaccharides from Citrus grandis L. Osbeck suppress inflammation and relieve chronic pharyngitis. |
