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JNK Polyclonal antibody

JNK Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 17337-1-AP

Print datasheet

Synonyms

c Jun N terminal kinase 2, JNK 55, JNK2, JNK2A, JNK2ALPHA, JNK2B, JNK2BETA, MAP kinase 9, MAPK 9, MAPK9, p54a, p54aSAPK, PRKM9, SAPK

验证数据展示

  • WB analysis of mouse brain using 17337-1-AP

    WB analysis of mouse brain using 17337-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17337-1-AP (JNK Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of SH-SY5Y using 17337-1-AP

    IF Staining of SH-SY5Y using 17337-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 17337-1-AP (JNK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse brain tissue
Positive IF detected inSH-SY5Y cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17337-1-AP targets JNK in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen JNK fusion protein Ag11372 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitogen-activated protein kinase 9
Synonyms c Jun N terminal kinase 2, JNK 55, JNK2, JNK2A, JNK2ALPHA, JNK2B, JNK2BETA, MAP kinase 9, MAPK 9, MAPK9, p54a, p54aSAPK, PRKM9, SAPK
Calculated Molecular Weight 424 aa, 48 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC032539
Gene Symbol MAPK9
Gene ID (NCBI) 5601
RRIDAB_2878388
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP45984
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

c-Jun N-terminal kinases (JNKs), first characterized as stress-activated members of the mitogen-activated protein kinase (MAPK) family, have shown their critical roles in the development of a number of cellular progress and diseases, including proliferation, embryonic development, apoptosis, diabetes, neurodegeneration and liver disease (PMID: 19900593). JNK1, JNK2, JNK3 also name as MAPK8, MAPK9, MAPK10, JNK1 and JNK2 are ubiquitously expressed, but JNK3 is expressed primarily in the brain (PMID: 27698029). This antibody can recognize JNK1, JNK2 and JNK3, we get bands around 44-55 kDa.

实验方案

Product Specific Protocols
WB protocol for JNK antibody 17337-1-APDownload protocol
IF protocol for JNK antibody 17337-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols