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验证数据展示

  • WB analysis of SKOV-3 using 11164-1-AP

    WB analysis of SKOV-3 using 11164-1-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 11164-1-AP (ERK5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 11164-1-AP

    IHC staining of human stomach using 11164-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11164-1-AP (ERK5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MAPK7 antibody included

ERK5 Polyclonal antibody

ERK5 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11164-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

ERK 5, MAPK7, MAPK 7, Extracellular signal-regulated kinase 5, ERK-5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inSKOV-3 cells, HEK-293 cells, mouse heart tissue, mouse testis tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy MAPK7 Ready-To-Use IHC Kit. MAPK7 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

11164-1-AP targets ERK5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag1648

Product name: Recombinant human MAPK7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC009963

Sequence: MAEPLKEEDGEDGSAEPPGPVKAEPAHTAASVAAKNLALLKARSFDVTFDVGDEYEIIETIGNGAYGVVSSARRRLTGQQVAIKKIPNAFDVVTNAKRTLRELKILKHFKHDNIIAIKDILRPTVPYGEFKSVYVVLDLMESDLHQIIHSSQPLTLEHVRYFLYQLLRGLKYMHSAQVIHRDLKPSNLLVNENCELKIGDFGMARGLCTSPAEHQYFMTEYVATRWYRAPELMLSLHEYTQAIDLWSVGCIFGEMLARRQLFPGKNYVHQLQLIMMVLGTPSPAVIQAVGAERVRAYIQSLPPRQPVPWETVYPGADRQALSLLGRMLRFEPSARISAAAALRHPFLAKY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitogen-activated protein kinase 7
别名 ERK 5, MAPK7, MAPK 7, Extracellular signal-regulated kinase 5, ERK-5
计算分子量 89 kDa
观测分子量 70 kDa
GenBank蛋白编号BC009963
基因名称 MAPK7
Gene ID (NCBI) 5598
RRIDAB_2877749
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13164
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ERK5 antibody 11164-1-APDownload protocol
IHC protocol for ERK5 antibody 11164-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell

Systematic discovery of mutation-directed neo-protein-protein interactions in cancer.

Authors - Xiulei Mo
View Article