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MAPK4 Polyclonal antibody

MAPK4 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

IHC, WB,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 26102-1-AP

Print datasheet

Synonyms

ERK 4, ERK3, Erk4, MAP kinase 4, MAP kinase isoform p63, MAPK 4, MAPK4, p63 MAPK, p63MAPK, PRKM4

验证数据展示

  • WB analysis of mouse brain using 26102-1-AP

    WB analysis of mouse brain using 26102-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 26102-1-AP (MAPK4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 26102-1-AP

    IHC staining of human breast cancer using 26102-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26102-1-AP (MAPK4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 26102-1-AP

    IHC staining of human breast cancer using 26102-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26102-1-AP (MAPK4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 26102-1-AP is MAPK4 Fusion Protein expressed in E. coli.

Tested Applications IHC, WB,ELISA
Cited ApplicationsWB, IF
Tested Reactivity human, mouse
Cited Reactivitymouse, pig
Immunogen MAPK4 fusion protein Ag22899 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitogen-activated protein kinase 4
Synonyms ERK 4, ERK3, Erk4, MAP kinase 4, MAP kinase isoform p63, MAPK 4, MAPK4, p63 MAPK, p63MAPK, PRKM4
Calculated Molecular Weight 587 aa, 66 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC050299
Gene Symbol MAPK4
Gene ID (NCBI) 5596
RRIDAB_2880378
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP31152
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MAPK4 antibody 26102-1-APDownload protocol
IHC protocol for MAPK4 antibody 26102-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Cell Biosci

Identification of atypical mitogen-activated protein kinase MAPK4 as a novel regulator in acute lung injury.

Authors - Ling Mao
View Article
pigWB

J Cell Physiol

MiR-127 attenuates adipogenesis by targeting MAPK4 and HOXC6 in porcine adipocytes.

Authors - Yun Gao
View Article
mouseWB

Front Pharmacol

An Integrated Proteomics and Bioinformatics Approach Reveals the Anti-inflammatory Mechanism of Carnosic Acid.

Authors - Li-Chao Wang
View Article