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MAPK4 Polyclonal antibody

MAPK4 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 18738-1-AP

Print datasheet

Synonyms

ERK 4, ERK3, Erk4, MAP kinase 4, MAP kinase isoform p63, MAPK 4, MAPK4, p63 MAPK, p63MAPK, PRKM4

验证数据展示

  • WB analysis of mouse brain using 18738-1-AP

    WB analysis of mouse brain using 18738-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 18738-1-AP (MAPK4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 18738-1-AP

    IHC staining of human heart using 18738-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18738-1-AP (MAPK4 Antibody) at dilution of 1:200 (under 40x lens).

  • IHC staining of human heart using 18738-1-AP

    IHC staining of human heart using 18738-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18738-1-AP (MAPK4 Antibody) at dilution of 1:200 (under 10x lens).

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18738-1-AP targets MAPK4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitogen-activated protein kinase 4
Synonyms ERK 4, ERK3, Erk4, MAP kinase 4, MAP kinase isoform p63, MAPK 4, MAPK4, p63 MAPK, p63MAPK, PRKM4
Calculated Molecular Weight 66 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC117216
Gene Symbol MAPK4
Gene ID (NCBI) 5596
RRIDAB_2878559
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP31152
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MAPK4 antibody 18738-1-APDownload protocol
IHC protocol for MAPK4 antibody 18738-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols