• Featured Product
  • KD/KO验证

MAPK12 Polyclonal antibody

MAPK12 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 20184-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA

MAP kinase 12, Extracellular signal-regulated kinase 6, ERK-6, ERK6, ERK 6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse heart tissue, human heart tissue, mouse skeletal muscle tissue, rat heart tissue
Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inmouse heart tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy MAPK12 Ready-To-Use IHC Kit. MAPK12 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20184-1-AP targets MAPK12 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 MAPK12 fusion protein Ag14073 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitogen-activated protein kinase 12
别名 MAP kinase 12, Extracellular signal-regulated kinase 6, ERK-6, ERK6, ERK 6
计算分子量 367 aa, 42 kDa
观测分子量 42 kDa
GenBank蛋白编号BC015741
基因名称 MAPK12
Gene ID (NCBI) 6300
RRIDAB_10665949
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP53778
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MAPK12 antibody 20184-1-APDownload protocol
IHC protocol for MAPK12 antibody 20184-1-APDownload protocol
IP protocol for MAPK12 antibody 20184-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Inflamm Regen

SPARC activates p38γ signaling to promote PFKFB3 protein stabilization and contributes to keloid fibroblast glycolysis

Authors - Yining Liu
humanWB,IHC

Tumour Biol

Clinicopathological significance of p38β, p38γ, and p38δ and its biological roles in esophageal squamous cell carcinoma.

Authors - Shutao Zheng
  • KD Validated
ratWB

BMC Endocr Disord

The role of MAPK11/12/13/14 (p38 MAPK) protein in dopamine agonist-resistant prolactinomas.

Authors - Shuman Wang
humanIHC

Clin Transl Oncol

A retrospective analysis of the clinicopathological features and prognostic value of MAPK12 protein expression in diffuse large B-cell lymphoma

Authors - Yue Liu
human,mouseWB,IHC

J Orthop Translat

Targeting the senescence-related genes MAPK12 and FOS to alleviate osteoarthritis

Authors - Nana Geng
Loading...