验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, human heart tissue, mouse skeletal muscle tissue |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 3 publications below |
| WB | See 5 publications below |
| IHC | See 1 publications below |
产品信息
17376-1-AP targets MAPK11 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | MAPK11 fusion protein Ag11184 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | mitogen-activated protein kinase 11 |
| 别名 | MAP kinase 11, MAP kinase p38 beta, MAPK 11, MAPK11, p38 2, P38B, p38Beta, P38BETA2, PRKM11, SAPK2, SAPK2B |
| 计算分子量 | 364 aa, 41 kDa |
| 观测分子量 | 42 kDa |
| GenBank蛋白编号 | BC027933 |
| 基因名称 | MAPK11 |
| Gene ID (NCBI) | 5600 |
| RRID | AB_2297482 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q15759 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MAPK11(Mitogen-activated protein kinase 11) is also named as PRKM11, SAPK2, SAPK2B,p38b and belongs to the MAP kinase subfamily.It is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors.In mouse brain, both p38a and p38b are present in neurons, while p38b is also expressed in glial cells(PMID:15748168).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MAPK11 antibody 17376-1-AP | Download protocol |
| IHC protocol for MAPK11 antibody 17376-1-AP | Download protocol |
| IF protocol for MAPK11 antibody 17376-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Theranostics Interleukin 33-mediated inhibition of A-type K+ channels induces sensory neuronal hyperexcitability and nociceptive behaviors in mice. | ||
Mol Pharmacol p38β MAPK signaling mediates exenatide-stimulated microglial β-endorphin expression.
| ||
Tumour Biol Clinicopathological significance of p38β, p38γ, and p38δ and its biological roles in esophageal squamous cell carcinoma.
| ||
BMC Endocr Disord The role of MAPK11/12/13/14 (p38 MAPK) protein in dopamine agonist-resistant prolactinomas.
| ||
J Hazard Mater Metastatic effects of environmental carcinogens mediated by MAPK and UPR pathways with an in vivo Drosophila Model |
