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验证数据展示

  • WB analysis using 20788-1-AP (same clone as 20788-1-PBS)

    WB analysis using 20788-1-AP (same clone as 20788-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20788-1-AP (MAP7D1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20788-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 20788-1-AP (same clone as 20788-1-PBS)

    IP experiment of HEK-293 using 20788-1-AP (same clone as 20788-1-PBS)

    IP result of anti-MAP7D1 (IP:20788-1-AP, 4ug; Detection:20788-1-AP 1:30000) with HEK-293 cells lysate 1470 ug. This data was developed using the same antibody clone with 20788-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 20788-1-AP (same clone as 20788-1-PBS)

    IHC staining of mouse heart using 20788-1-AP (same clone as 20788-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20788-1-AP (MAP7D1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20788-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MAP7D1 antibody included
  • IHC staining of mouse skeletal muscle using 20788-1-AP (same clone as 20788-1-PBS)

    IHC staining of mouse skeletal muscle using 20788-1-AP (same clone as 20788-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20788-1-AP (MAP7D1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20788-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MAP7D1 antibody included
  • IF Staining of U-251 using 20788-1-AP (same clone as 20788-1-PBS)

    IF Staining of U-251 using 20788-1-AP (same clone as 20788-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using MAP7D1 antibody (20788-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 20788-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 20788-1-AP (same clone as 20788-1-PBS)

    IF Staining of HeLa using 20788-1-AP (same clone as 20788-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MAP7D1 antibody (20788-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 20788-1-PBS in a different storage buffer formulation.

MAP7D1 Polyclonal antibody, PBS Only

MAP7D1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 20788-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

Arginine/proline-rich coiled-coil domain-containing protein 1, KIAA1187, MAP7 domain-containing protein 1, PARCC1, PP2464

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® MAP7D1 Ready-To-Use IHC Kit. MAP7D1 primary antibody included.

产品信息

20788-1-PBS targets MAP7D1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag14385

Product name: Recombinant human MAP7D1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 591-841 aa of BC003083

Sequence: MAGTTDREEATRLLAEKRRQAREQREREEQERRLQAERDKRMREEQLAREAEARAEREAEARRREEQEAREKAQAEQEEQERLQKQKEEAEARSREEAERQRLEREKHFQQQEQERQERRKRLEEIMKRTRKSEVSETKKQDSKEANANGSSPEPVKAVEARSPGLQKEAVQKEEPIPQEPQWSLPSKELPASLVNGLQPLPAHQENGFSTNGPSGDKSLSRTPETLLPFAEAEAFLKKAVVQSPQVTEVL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 MAP7 domain containing 1
别名 Arginine/proline-rich coiled-coil domain-containing protein 1, KIAA1187, MAP7 domain-containing protein 1, PARCC1, PP2464
计算分子量 841 aa, 93 kDa
观测分子量 120-130 kDa
GenBank蛋白编号BC003083
基因名称 MAP7D1
Gene ID (NCBI) 55700
RRIDAB_3669347
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ3KQU3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MAP7D1 also known as RPRC1, PARCC1, belongs to the MAP7 family. The MAP7 (Microtubule Associated Protein 7) protein family, consisting of four members, MAP7, MAP7D1, and MAP7D2, MAP7D3, is the microtubule-associated protein involved in various cellular processes regulating microtubule dynamics, organization, and stability(PMID: 28980356). MAP7D1 exhibits the highest conservation with MAP7 and was recently identified as a phosphorylation substrate of DCLK1 in cortical neurons. MAP7D1 is required to maintain MT acetylation, which is enriched in stable MTs(PMID: 35470240). Consistent with the literature, the apparent molecular mass of MAP7D1 detected by Western blot was 120-130 kDa (PMID: 35470240, 37550720).

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