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验证数据展示

  • WB analysis using 25717-1-AP (same clone as 25717-1-PBS)

    WB analysis using 25717-1-AP (same clone as 25717-1-PBS)

    mouse retina tissue were subjected to SDS PAGE followed by western blot with 25717-1-AP (MAP6/STOP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25717-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse cerebellum using 25717-1-AP (same clone as 25717-1-PBS)

    IHC staining of mouse cerebellum using 25717-1-AP (same clone as 25717-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25717-1-AP (MAP6/STOP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25717-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse cerebellum using 25717-1-AP (same clone as 25717-1-PBS)

    IHC staining of mouse cerebellum using 25717-1-AP (same clone as 25717-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25717-1-AP (MAP6/STOP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25717-1-PBS in a different storage buffer formulation.

  • IF Staining of PC-12 using 25717-1-AP (same clone as 25717-1-PBS)

    IF Staining of PC-12 using 25717-1-AP (same clone as 25717-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed PC-12 cells using MAP6/STOP antibody (25717-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25717-1-PBS in a different storage buffer formulation.

MAP6/STOP Polyclonal antibody, PBS Only

MAP6/STOP Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 25717-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

25717-1-PBS targets MAP6/STOP in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag22471

Product name: Recombinant human MAP6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 144-484 aa of BC139780

Sequence: GQGPMVQEPLKKQGSVVPGPPKDLGPMIPLPVKDQDHTVPEPLKNESPVISAPVKDQGPSVPVPPKNQSPMVPAKVKDQGSVVPESLKDQGPRIPEPVKNQAPMVPAPVKDEGPMVSASVKDQGPMVSAPVKDQGPIVPAPVKGEGPIVPAPVKDEGPMVSAPIKDQDPMVPEHPKDESAMATAPIKNQGSMVSEPVKNQGLVVSGPVKDQDVVVPEHAKVHDSAVVAPVKNQGPVVPESVKNQDPILPVLVKDQGPTVLQPPKNQGRIVPEPLKNQVPIVPVPLKDQDPLVPVPAKDQGPAVPEPLKTQGPRDPQLPTVSPLPRVMIPTAPHTEYIESSP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 microtubule-associated protein 6
别名
计算分子量 813 aa, 87 kDa
观测分子量120 kDa
GenBank蛋白编号BC139780
基因名称 MAP6
Gene ID (NCBI) 4135
RRIDAB_3085819
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96JE9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MAP6, also named STOP protein, has been isolated from brain preparations but is also present in other tissues such as the skeletal muscles and heart. MAP6 is a calmodulin-binding and calmodulin-regulated protein that is involved in microtubule stabilization. Different isoforms of STOP protein arise from alternative mRNA splicing and transcription initiation sites using a single gene. The neuronal MAP6 isoforms N-STOP (120 kDa) and E-STOP (80 kDa), the astrocyte MAP6 isoform A-STOP (60 KDa), and a fibroblastic MAP6 isoform F-STOP (48 kDa) have been identified.

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