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验证数据展示

  • WB analysis of mouse ovary using 12827-1-AP

    WB analysis of mouse ovary using 12827-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 12827-1-AP (MAP4K5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 12827-1-AP

    IHC staining of human ovary tumor using 12827-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12827-1-AP (MAP4K5 antibody) at dilution of 1:100 (under 10x lens).

MAP4K5 Polyclonal antibody

MAP4K5 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12827-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

GCKR, KHS, KHS1, MAP4K5, MAPKKKK5, MEK kinase kinase 5, MEKKK 5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse ovary tissue, HeLa cells, K-562 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12827-1-AP targets MAP4K5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MAP4K5 fusion protein Ag3547 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitogen-activated protein kinase kinase kinase kinase 5
别名 GCKR, KHS, KHS1, MAP4K5, MAPKKKK5, MEK kinase kinase 5, MEKKK 5
计算分子量 846 aa, 95 kDa
观测分子量 58 kDa, 95 kDa, 120 kDa
GenBank蛋白编号BC036013
基因名称 MAP4K5
Gene ID (NCBI) 11183
RRIDAB_2140986
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y4K4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MAP4K5 antibody 12827-1-APDownload protocol
IHC protocol for MAP4K5 antibody 12827-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols