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验证数据展示

  • WB analysis of mouse ovary using 12827-1-AP

    WB analysis of mouse ovary using 12827-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 12827-1-AP (MAP4K5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 12827-1-AP

    IHC staining of human ovary tumor using 12827-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12827-1-AP (MAP4K5 antibody) at dilution of 1:100 (under 10x lens).

MAP4K5 Polyclonal antibody

MAP4K5 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12827-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

GCKR, KHS, KHS1, MAP4K5, MAPKKKK5, MEK kinase kinase 5, MEKKK 5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse ovary tissue, HeLa cells, K-562 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12827-1-AP targets MAP4K5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3547

Product name: Recombinant human MAP4K5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 5-274 aa of BC036013

Sequence: LRPAADILRRNPQQDYELVQRVGSGTYGDVYKARNVHTGELAAVKIIKLEPGDDFSLIQQEIFMVKECKHCNIVAYFGSYLSREKLWICMEYCGGGSLQDIYHVTGPLSELQIAYVCRETLQGLAYLHTKGKMHRDIKGANILLTDHGDVKLADFGVAAKITATIAKRKSFIGTPYWMAPEVAAVEKNGGYNQLCDIWAVGITAIELGELQPPMFDLHPMRALFLMSKSNFQPPKLKDKTKWSSTFHNFVKIALTKNPKKRPTAERLLTH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitogen-activated protein kinase kinase kinase kinase 5
别名 GCKR, KHS, KHS1, MAP4K5, MAPKKKK5, MEK kinase kinase 5, MEKKK 5
计算分子量 846 aa, 95 kDa
观测分子量 58 kDa, 95 kDa, 120 kDa
GenBank蛋白编号BC036013
基因名称 MAP4K5
Gene ID (NCBI) 11183
RRIDAB_2140986
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y4K4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MAP4K5 antibody 12827-1-APDownload protocol
IHC protocol for MAP4K5 antibody 12827-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols