验证数据展示
经过测试的应用
| Positive WB detected in | HK-2 cells, Jurkat cells, PC-3 cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human skeletal muscle tissue, human placenta tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy MAP4K4 Ready-To-Use IHC Kit. MAP4K4 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
55247-1-AP targets MAP4K4 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | mitogen-activated protein kinase kinase kinase kinase 4 |
| 别名 | EC:2.7.11.1, FLH21957, HGK, HPK/GCK like kinase HGK, HPK/GCK-like kinase HGK |
| 计算分子量 | 151 kDa |
| 观测分子量 | 130-160 kDa |
| GenBank蛋白编号 | NM_004834 |
| 基因名称 | MAP4K4 |
| Gene ID (NCBI) | 9448 |
| RRID | AB_10836939 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O95819 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MAP4K4, also named as RAB8IPL1, GLK and MEKKK4, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and STE20 subfamily. It may play a role in the response to environmental stress. MAP4K4 appears to act upstream of the JUN N-terminal pathway. This antibody is specific to MAP4K4.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for MAP4K4 antibody 55247-1-AP | Download protocol |
| IP protocol for MAP4K4 antibody 55247-1-AP | Download protocol |
| WB protocol for MAP4K4 antibody 55247-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Phytomedicine Artesunate improves glucose and lipid metabolism in db/db mice by regulating the metabolic profile and the MAPK/PI3K/Akt signalling pathway | ||
Anal Chem Parallel-Reaction-Monitoring-Based Proteome-Wide Profiling of Differential Kinase Protein Expression during Prostate Cancer Metastasis in Vitro. | ||
J Proteome Res Global Phosphoproteomic Analysis Reveals Significant Metabolic Reprogramming in the Termination of Liver Regeneration in Mice. | ||
Neural Regen Res MAP4K4 induces early blood-brain barrier damage in a murine subarachnoid hemorrhage model.
| ||
PeerJ Construction and validation of a novel prognostic signature of microRNAs in lung adenocarcinoma. |
