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MAP4K1 Polyclonal antibody

MAP4K1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 23950-1-AP

Print datasheet

Synonyms

HPK1, MAP4K1, MEK kinase kinase 1, MEKKK 1

验证数据展示

  • WB analysis of Raji using 23950-1-AP

    WB analysis of Raji using 23950-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 23950-1-AP (MAP4K1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 23950-1-AP

    IHC staining of human tonsillitis using 23950-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 23950-1-AP (MAP4K1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of Jurkat using 23950-1-AP

    IP experiment of Jurkat using 23950-1-AP

    IP result of anti-MAP4K1 (IP:23950-1-AP, 4ug; Detection:23950-1-AP 1:1000) with Jurkat cells lysate 3200ug.

  • WB analysis using 23950-1-AP

    WB analysis using 23950-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23950-1-AP (MAP4K1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 23950-1-AP

    IHC staining of human liver using 23950-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 23950-1-AP (MAP4K1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 23950-1-AP

    IHC staining of human liver using 23950-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 23950-1-AP (MAP4K1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inJurkat cells, Raji cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman tonsillitis tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

23950-1-AP targets MAP4K1 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen MAP4K1 fusion protein Ag20221 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitogen-activated protein kinase kinase kinase kinase 1
Synonyms HPK1, MAP4K1, MEK kinase kinase 1, MEKKK 1
Calculated Molecular Weight 833 aa, 91 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC152994
Gene Symbol MAP4K1
Gene ID (NCBI) 11184
RRIDAB_2879372
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ92918
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAP4K1(Mitogen-activated protein kinase kinase kinase kinase 1) is also named as MEKKK 1, HPK1 and belongs to the protein kinase superfamily. It may function as a novel tumor suppressor and its loss plays a critical role in pancreatic cancer. MAP4K1 regulates stress responses, proliferation, and apoptosis in hematopoietic cells. This protein has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for MAP4K1 antibody 23950-1-APDownload protocol
IHC protocol for MAP4K1 antibody 23950-1-APDownload protocol
IP protocol for MAP4K1 antibody 23950-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Signal

SPIB acts as a tumor suppressor by activating the NFkB and JNK signaling pathways through MAP4K1 in colorectal cancer cells.

Authors - Xunping Zhao
View Article
humanWB

Clin Exp Med

An HPK1 inhibitor enhanced the tumour response to anti-PD-1 immunotherapy in non-Hodgkin's lymphoma

Authors - Lin Yang
View Article
humanWB

Nat Commun

DNA framework-engineered chimeras platform enables selectively targeted protein degradation

Authors - Li Zhou
View Article