验证数据展示
经过测试的应用
| Positive WB detected in | NIH/3T3 cells, K-562 cells, SKOV-3 cells, COLO 320 cells, A375 cells, A431 cells, CHO cells, MCF-7 cells, HeLa cells, mouse heart tissue, A2780 cells |
| Positive IHC detected in | human prostate cancer tissue, human brain tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | NIH/3T3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12330-2-AP targets TAK1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, hamster samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human, mouse, hamster |
| 文献引用反应性 | human, mouse, rat, pig, fish |
| 免疫原 | TAK1 fusion protein Ag3041 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | mitogen-activated protein kinase kinase kinase 7 |
| 别名 | MAP3K7, TGF beta activated kinase 1, Mitogen-activated protein kinase kinase kinase 7, mitogen activated protein kinase kinase kinase 7, MAP3K 7 |
| 计算分子量 | 579 aa, 64 kDa |
| 观测分子量 | 64-67 kDa, 75-80 kDa |
| GenBank蛋白编号 | BC017715 |
| 基因名称 | TAK1 |
| Gene ID (NCBI) | 6885 |
| RRID | AB_2140101 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O43318 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Transforming growth factor-β (TGF-β)-activated kinase 1 (TAK1), also known as MAP3K7-mitogen-activated protein kinase kinase kinase 7- is a serine/threonine protein kinase triggering phosphorylation of AMP-activated protein kinase (AMPK) and playing a role in the signal transduction of TGF-β, bone morphogenetic proteins (BMPs), and various proinflammatory mediators.
What is the molecular weight of TAK1? Are there any isoforms of TAK1?
The molecular weight of full-length TAK1 is 55 kDa. There are four splicing variants of TAK1. TAK1-B represents the full-length variant, while TAK1-A, TAK1-C, and TAK1-D lack exons 12 and/or 16. TAK-1 variants differ in their expression levels across tissues (PMID: 11118615).
Is TAK1 post-translationally modified?
TAK1 kinase activity is modulated by interacting with TAK1-binding proteins (TABs). Binding to TABs triggers autophosphorylation of TAK1 and depends on polyubiquitination of receptor-interacting protein 1 (RIP1) (PMID: 8638164 and 11460167). Additionally, TAK1 can be ubiquitinated, acetylated, and O-glycosylated, which impacts its activity (PMID: 28106845).
What is the subcellular localization of TAK1?
TAK1 is present in the cytoplasm.
What is the tissue expression pattern of TAK1?
TAK1 is ubiquitously expressed.
How can TAK1 activity be monitored?
TAK1 requires phosphorylation for its activity. TAK1 autophosphorylation in the presence of TBPs occurs on Thr178, Thr184, Thr187, and Ser192 residues. The most commonly used phosphorylation readout is Thr187 phosphorylation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TAK1 antibody 12330-2-AP | Download protocol |
| IHC protocol for TAK1 antibody 12330-2-AP | Download protocol |
| IF protocol for TAK1 antibody 12330-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
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Diabetes Hepatic P38 Activation Modulates Systemic Metabolism Through Fgf21-Mediated Interorgan Communication. | ||
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