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MEKK2 Polyclonal antibody

MEKK2 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 55106-1-AP

Print datasheet

Synonyms

MAP3K2, MAPK/ERK kinase kinase 2, MAPKKK2, MEK kinase 2, MEKK 2, MEKK2, MEKK2B

验证数据展示

  • WB analysis using 55106-1-AP

    WB analysis using 55106-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55106-1-AP (MEKK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 55106-1-AP

    IP experiment of HEK-293 using 55106-1-AP

    IP result of anti-MEKK2 (IP:55106-1-AP, 4ug; Detection:55106-1-AP 1:300) with HEK-293 cells lysate 3200ug.

经过测试的应用

Positive WB detected inK-562 cells, MCF-7 cells
Positive IP detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55106-1-AP targets MEKK2 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IP, WB, ELISA
Cited ApplicationsWB, IP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitogen-activated protein kinase kinase kinase 2
Synonyms MAP3K2, MAPK/ERK kinase kinase 2, MAPKKK2, MEK kinase 2, MEKK 2, MEKK2, MEKK2B
Calculated Molecular Weight 70 kDa
Observed Molecular Weight 65-75 kDa
GenBank Accession NumberNM_006609
Gene Symbol MAP3K2
Gene ID (NCBI) 10746
RRIDAB_11064604
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y2U5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAP3K2, also named as MEKK2 and MEKK2B, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and MAPK kinase kinase subfamily. It is a component of a protein kinase signal transduction cascade. MAP3K2 mediates activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators. The antibody is specific to MAP3K2.

实验方案

Product Specific Protocols
WB protocol for MEKK2 antibody 55106-1-APDownload protocol
IP protocol for MEKK2 antibody 55106-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIP,WB

Mol Cancer

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Authors - Runjie Song
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