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MAP3K2 Polyclonal antibody

MAP3K2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 29265-1-AP

Print datasheet

Synonyms

MAP3K2, MAPK/ERK kinase kinase 2, MAPKKK2, MEK kinase 2, MEKK 2, MEKK2, MEKK2B

验证数据展示

  • WB analysis using 29265-1-AP

    WB analysis using 29265-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29265-1-AP (MAP3K2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 29265-1-AP

    IHC staining of human breast cancer using 29265-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29265-1-AP (MAP3K2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 29265-1-AP

    IHC staining of human breast cancer using 29265-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29265-1-AP (MAP3K2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, K-562 cells, MCF-7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

29265-1-AP targets MAP3K2 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity Human
Cited Reactivityhuman
Immunogen MAP3K2 fusion protein Ag30886 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitogen-activated protein kinase kinase kinase 2
Synonyms MAP3K2, MAPK/ERK kinase kinase 2, MAPKKK2, MEK kinase 2, MEKK 2, MEKK2, MEKK2B
Calculated Molecular Weight 70 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberNM_006609
Gene Symbol MAP3K2
Gene ID (NCBI) 10746
RRIDAB_2918263
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y2U5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAP3K2, also named as MEKK2 and MEKK2B, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and MAPK kinase kinase subfamily. It is a component of a protein kinase signal transduction cascade. MAP3K2 mediates activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators. The antibody is specific to MAP3K2.

实验方案

Product Specific Protocols
WB protocol for MAP3K2 antibody 29265-1-APDownload protocol
IHC protocol for MAP3K2 antibody 29265-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Ther Nucleic Acids

Concurrent targeting of MAP3K3 and BRD4 by miR-3140-3p overcomes acquired resistance to BET inhibitors in neuroblastoma cells.

Authors - Chang Liu
View Article