- Featured Product
- KD/KO Validated
MEK3 Polyclonal antibody
MEK3 Polyclonal Antibody for IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells |
| Positive IP detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 3 publications below |
| WB | See 9 publications below |
产品信息
13898-1-AP targets MEK3 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IP, WB, ELISA |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | MEK3 fusion protein Ag4861 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | mitogen-activated protein kinase kinase 3 |
| Synonyms | MAP kinase kinase 3, MAP2K3, MAPK/ERK kinase 3, MAPKK 3, MAPKK3, MEK 3, MEK3, MKK3, PRKMK3 |
| Calculated Molecular Weight | 347 aa, 39 kDa |
| Observed Molecular Weight | 37-40 kDa |
| GenBank Accession Number | BC032478 |
| Gene Symbol | MAP2K3 |
| Gene ID (NCBI) | 5606 |
| RRID | AB_10642959 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P46734 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MEK3 antibody 13898-1-AP | Download protocol |
| IHC protocol for MEK3 antibody 13898-1-AP | Download protocol |
| IP protocol for MEK3 antibody 13898-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biomed Pharmacother Identification of MAP Kinase Kinase 3 as a protein target of myricetin in non-small cell lung cancer cells
| ||
Cell Mol Life Sci HERC2 deficiency activates C-RAF/MKK3/p38 signalling pathway altering the cellular response to oxidative stress | ||
Food Funct L-Theanine mediates the p38MAPK signaling pathway to alleviate heat-induced oxidative stress and inflammation in mice. | ||
Sci Rep The ubiquitin ligase HERC1 regulates cell migration via RAF-dependent regulation of MKK3/p38 signaling.
| ||
Mol Immunol Spiroplasma eriocheiris induces mouse 3T6-Swiss albino cell apoptosis that associated with the infection mechanism. | ||
