验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, human brain tissue, HL-60 cells, HeLa cells, Jurkat cells, SH-SY5Y cells, mouse brain tissue, rat brain tissue |
| Positive IP detected in | HL-60 cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Jurkat cells, SH-SY5Y cells |
Planning an IHC experiment? We recommend our IHCeasy MAP2K2 Ready-To-Use IHC Kit. MAP2K2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11049-1-AP targets MEK1/2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | MEK1/2 fusion protein Ag1481 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | mitogen-activated protein kinase kinase 2 |
| 别名 | MAP2K2, MEK2, MAPK/ERK kinase 2, MAP kinase kinase 2, ERK activator kinase 2 |
| 计算分子量 | 44 kDa |
| 观测分子量 | 44-47 kDa |
| GenBank蛋白编号 | BC018645 |
| 基因名称 | MEK2 |
| Gene ID (NCBI) | 5605 |
| RRID | AB_2140649 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P36507 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MEK2(MAPK/ERK kinase 2) is also named as MAP2K2, MKK2, PRKMK2 and belongs to the MAP kinase kinase subfamily. MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). MEK1 and MEK2 are closely related, dual-specificity tyrosine/threonine protein kinases found in the Ras/Raf/MEK/ERK mitogen-activated protein kinase (MAPK) signaling pathway. 11049-1-AP can recognize both the MEK2 and MEK1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MEK1/2 antibody 11049-1-AP | Download protocol |
| IHC protocol for MEK1/2 antibody 11049-1-AP | Download protocol |
| IF protocol for MEK1/2 antibody 11049-1-AP | Download protocol |
| IP protocol for MEK1/2 antibody 11049-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Exp Mol Med Helicobacter pylori CagA-mediated ether lipid biosynthesis promotes ferroptosis susceptibility in gastric cancer | ||
ACS Appl Mater Interfaces Near-Infrared Light-Responsive Nanoinhibitors for Tumor Suppression through Targeting and Regulating Anion Channels. | ||
Cell Rep Targeting CXCR1 alleviates hyperoxia-induced lung injury through promoting glutamine metabolism | ||
J Med Chem Identification of a Novel TAR RNA-Binding Protein 2 Modulator with Potential Therapeutic Activity against Hepatocellular Carcinoma. | ||
