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验证数据展示

  • WB analysis of HEK-293 using 21296-1-AP (same clone as 21296-1-PBS)

    WB analysis of HEK-293 using 21296-1-AP (same clone as 21296-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 21296-1-AP (MANEA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21296-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 21296-1-AP (same clone as 21296-1-PBS)

    IHC staining of human breast cancer using 21296-1-AP (same clone as 21296-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21296-1-AP (MANEA Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 21296-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 21296-1-AP (same clone as 21296-1-PBS)

    IHC staining of human breast cancer using 21296-1-AP (same clone as 21296-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21296-1-AP (MANEA Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 21296-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 21296-1-AP (same clone as 21296-1-PBS)

    IF Staining of HEK-293 using 21296-1-AP (same clone as 21296-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using MANEA antibody (21296-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 21296-1-PBS in a different storage buffer formulation.

MANEA Polyclonal antibody, PBS Only

MANEA Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 21296-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Glycoprotein endo-alpha-1,2-mannosidase, Endomannosidase, Endo-alpha mannosidase, Endo alpha mannosidase, ENDO

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

21296-1-PBS targets MANEA in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag15847

Product name: Recombinant human MANEA protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 29-109 aa of BC146671

Sequence: LRPNTATFGAPFGLDLLPELHQRTIHLGKNFDFQKSDRINSETNTKNLKSVEITMKPSKASELNLDELPPLNNYLHVFYYS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mannosidase, endo-alpha
别名 Glycoprotein endo-alpha-1,2-mannosidase, Endomannosidase, Endo-alpha mannosidase, Endo alpha mannosidase, ENDO
计算分子量 462 aa, 54 kDa
观测分子量 54 kDa
GenBank蛋白编号BC146671
基因名称 MANEA
Gene ID (NCBI) 79694
RRIDAB_2878836
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5SRI9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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