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验证数据展示

  • WB analysis using 17505-1-AP

    WB analysis using 17505-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17505-1-AP (MAK16 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 17505-1-AP

    IF Staining of A431 using 17505-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using MAK16 antibody (17505-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

MAK16 Polyclonal antibody

MAK16 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 17505-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

RBM13, Protein RBM13, Protein MAK16 homolog, NNP78

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, HepG2 cells
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17505-1-AP targets MAK16 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MAK16 fusion protein Ag11705 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 MAK16 homolog (S. cerevisiae)
别名 RBM13, Protein RBM13, Protein MAK16 homolog, NNP78
计算分子量 300 aa, 35 kDa
观测分子量 48 kDa
GenBank蛋白编号BC050528
基因名称 MAK16
Gene ID (NCBI) 84549
RRIDAB_2281504
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BXY0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MAK16 antibody 17505-1-APDownload protocol
IF protocol for MAK16 antibody 17505-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols