验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, NIH/3T3 cells, HuH-7 cells, rat brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
27994-1-AP targets MAGT1 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human, Mouse, Rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag27416 Product name: Recombinant human MAGT1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 266-335 aa of BC060842 Sequence: VAETHIVLLFNGGVTLGMVLLCEAATSDMDIGKRKIMCVAGIGLVVLFFSWMLSIFRSKYHGYPYSFLMS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | magnesium transporter 1 |
| 别名 | bA217H1.1, DKFZp564K142, FLJ14726, IAG2, IAP, magnesium transporter 1, MAGT1, OST3B, PRO0756, UNQ628/PRO1244 |
| 计算分子量 | 335 aa, 38 kDa |
| 观测分子量 | 45-50 kDa |
| GenBank蛋白编号 | BC060842 |
| 基因名称 | MAGT1 |
| Gene ID (NCBI) | 84061 |
| RRID | AB_2881033 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H0U3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MAGT1 is a mammalian Mg2+-selective transporter being required for cellular magnesium uptake and vertebrate embryonic development. It possesses five putative transmembrane (TM) regions with a cleavage site, a N-glycosylation site, and a number of phosphorylation sites. Recently mutations in MAGT1 has been found to be asscociated with a novel X-linked human immunodeficiency. The MAGT1 protein was undetectable in the patients' cells by western blot or immunofluorescent cell surface staining (Nature 475, 471-6). 27994-1-AP antibody detects the glycosylated protein around 45-50 kDa in SDS-PAGE. (PMID: 28720733, 27383987)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MAGT1 antibody 27994-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
