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Species Reactivity
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Species Reactivity
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Conjugate
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Compatible Species
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验证数据展示

  • WB analysis using 32867-1-AP

    WB analysis using 32867-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32867-1-AP (MAGOHB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

MAGOHB Polyclonal antibody

MAGOHB Polyclonal Antibody for WB, ELISA
Cat No. 32867-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

MAGOH2, Protein mago nashi homolog 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, Raji cells, Ramos cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32867-1-AP targets MAGOHB in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag37296

Product name: Recombinant human MAGOHB protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-116 aa of BC010905

Sequence: MAVASDFYLRYYVGHKGKFGHEFLEFEFRPDGKLRYANNSNYKNDVMIRKEAYVHKSVMEELKRIIDDSEITKEDDALWPPPDRVGRQELEIVIGDEHISFTTSKIGSLIDVNQSK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mago-nashi homolog B (Drosophila)
别名 MAGOH2, Protein mago nashi homolog 2
计算分子量 17 kDa
观测分子量15 kDa
GenBank蛋白编号BC010905
基因名称 MAGOHB
Gene ID (NCBI) 55110
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ96A72
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mago Homolog B, Exon Junction Complex Subunit (MAGOHB), is a key component of the exon junction complex (EJC), which is crucial for pre-mRNA splicing, mRNA transport, and nonsense-mediated decay (NMD). It plays a redundant role with MAGOH in the EJC and is required for proper mRNA processing and translation (PMID: 23917022). Additionally, MagoHB is involved in multiple biological processes, including neurogenesis, brain development, cell cycle regulation, and apoptosis (PMID: 37294214).

实验方案

Product Specific Protocols
WB protocol for MAGOHB antibody 32867-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols