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验证数据展示

  • WB analysis using 12347-1-AP (same clone as 12347-1-PBS)

    WB analysis using 12347-1-AP (same clone as 12347-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12347-1-AP (MAGOH antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12347-1-PBS in a different storage buffer formulation.

  • IP experiment of K-562 using 12347-1-AP (same clone as 12347-1-PBS)

    IP experiment of K-562 using 12347-1-AP (same clone as 12347-1-PBS)

    IP result of anti-MAGOH (IP:12347-1-AP, 3ug; Detection:12347-1-AP 1:500) with K-562 cells lysate 2400ug. This data was developed using the same antibody clone with 12347-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 12347-1-AP (same clone as 12347-1-PBS)

    IHC staining of human ovary tumor using 12347-1-AP (same clone as 12347-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12347-1-AP (MAGOH antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12347-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MAGOH antibody included
  • IHC staining of human ovary tumor using 12347-1-AP (same clone as 12347-1-PBS)

    IHC staining of human ovary tumor using 12347-1-AP (same clone as 12347-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12347-1-AP (MAGOH antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12347-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MAGOH antibody included

MAGOH Polyclonal antibody, PBS Only

MAGOH Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 12347-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

MAGOHA

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy MAGOH Ready-To-Use IHC Kit. MAGOH primary antibody included.

产品信息

12347-1-PBS targets MAGOH in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3004

Product name: Recombinant human MAGOH protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-146 aa of BC018211

Sequence: MESDFYLRYYVGHKGKFGHEFLEFEFRPDGKLRYANNSNYKNDVMIRKEAYVHKSVMEELKRIIDDSEITKEDDALWPPPDRVGRQELEIVIGDEHISFTTSKIGSLIDVNQSKDPEGLRVFYYLVQDLKCLVFSLIGLHFKIKPI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mago-nashi homolog, proliferation-associated (Drosophila)
别名 MAGOHA
计算分子量 146 aa, 17 kDa
观测分子量 17 kDa
GenBank蛋白编号BC018211
基因名称 MAGOH
Gene ID (NCBI) 4116
RRIDAB_2265988
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP61326
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MAGOH, belonging to the mago nashi family, is a component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). MAGOH regulates the transcriptional activation of STAT3 by interfering complex formation between STAT3 and a core EJC component Y14.

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