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验证数据展示

  • WB analysis of Jurkat using 16809-1-AP (same clone as 16809-1-PBS)

    WB analysis of Jurkat using 16809-1-AP (same clone as 16809-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 16809-1-AP (MAGED4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16809-1-PBS in a different storage buffer formulation.

  • IHC staining of human gliomas using 16809-1-AP (same clone as 16809-1-PBS)

    IHC staining of human gliomas using 16809-1-AP (same clone as 16809-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 16809-1-AP (MAGED4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16809-1-PBS in a different storage buffer formulation.

  • IHC staining of human gliomas using 16809-1-AP (same clone as 16809-1-PBS)

    IHC staining of human gliomas using 16809-1-AP (same clone as 16809-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 16809-1-AP (MAGED4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16809-1-PBS in a different storage buffer formulation.

MAGED4 Polyclonal antibody, PBS Only

MAGED4 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 16809-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

16809-1-PBS targets MAGED4 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag10329

Product name: Recombinant human MAGED4 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 1-350 aa of BC081566

Sequence: MAEGSFSVQSESYSVEDMDEGSDEVGEEEMVEGNDYEEFGAFGGYGTLTSFDIHILRAFGSLGPGLRILSNEPWELENPVLAQTLVEALQLDPETLANETAARAANVARAAASNRAARAAAAAARTAFSQVVASHRVATPQVSGEDTQPTTYAAEAQGPTPEPPLASPQTSQMLVTSKMAAPEAPATSAQSQTGSPAQEAATEGPSSACAFSQAPCAREVDANRPSTAFLGQNDVFDFTQPAGVSGMAFPRPKRPAPAQEAATEGPSAASGVPQTGPGREVAATRPKTTKSGKALAKTRWVEPQNVVAAAAAKAKMATSIPEPEGAAAATAQHSAEPWARMGGKRTKKSK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 melanoma antigen family D, 4
别名
计算分子量 741 aa, 81 kDa
观测分子量 90-100 kDa
GenBank蛋白编号BC081566
基因名称 MAGED4
Gene ID (NCBI) 728239
RRIDAB_2138678
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96JG8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MAGED4, originally termed MAGEE1, is a member of MAGE (Melanoma associated antigen) super-family identified by serial analysis of gene expression (SAGE) technique (PMID: 11406556; 24966945). Three isoforms (MAGED4a, b, and c) were produced by alternative splice in the process of gene transcription (PMID: 11406556). This antibody raised against 1-350 aa of human MAGED4 protein detects a band of 90-100 kDa, which is larger than the calculated molecular weight. The slower electrophoretic mobility may be due to abnormal spatial conformation of MAGED4 protein caused by abundance of proline in composition (PMID: 24966945).

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