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验证数据展示

  • WB analysis using 12786-2-AP

    WB analysis using 12786-2-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12786-2-AP (MAGEB4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 12786-2-AP

    IF Staining of HepG2 using 12786-2-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MAGEB4 antibody (12786-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • IP experiment of K-562 using 12786-2-AP

    IP experiment of K-562 using 12786-2-AP

    IP result of anti-MAGEB4 (IP:12786-2-AP, 4ug; Detection:12786-2-AP 1:300) with K-562 cells lysate 4000 ug.

MAGEB4 Polyclonal antibody

MAGEB4 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 12786-2-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, IP, ELISA

别名

Melanoma-associated antigen B4, MAGE-B4 antigen

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inmouse testis tissue, human testis tissue, K-562 cells
Positive IP detected inK-562 cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:600-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12786-2-AP targets MAGEB4 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag3502

Product name: Recombinant human MAGEB4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-346 aa of BC032852

Sequence: MPRGQKSKLRAREKRQRTRGQTQDLKVGQPTAAEKEESPSPSSSVLRDTASSSLAFGIPQEPQREPPTTSAAAAMSCTGSDKGDESQDEENASSSQASTSTERSLKDSLTRKTKMLVQFLLYKYKMKEPTTKAEMLKIISKKYKEHFPEIFRKVSQRTELVFGLALKEVNPTTHSYILVSMLGPNYGNQSSAWTLPRNGLLMPLLSVIFLNGNCAREEEIWEFLNMLGIYDGKRHLIFGEPRKLITQDLVQEKYLEYQQVPNSDPPRYQFLWGPRAHAETSKMKVLEFLAKVNDTTPNNFPLLYEEALRDEEERAGARPRVAARRGTTAMTSAYSRATSSSSSQPM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 melanoma antigen family B, 4
别名 Melanoma-associated antigen B4, MAGE-B4 antigen
计算分子量 346 aa, 39 kDa
观测分子量 40 kDa
GenBank蛋白编号BC032852
基因名称 MAGEB4
Gene ID (NCBI) 4115
RRIDAB_2138418
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15481
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for MAGEB4 antibody 12786-2-APDownload protocol
IP protocol for MAGEB4 antibody 12786-2-APDownload protocol
WB protocol for MAGEB4 antibody 12786-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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