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验证数据展示

  • WB analysis of HepG2 using 12400-1-AP

    WB analysis of HepG2 using 12400-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12400-1-AP (MAGEB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 12400-1-AP

    IF Staining of HepG2 using 12400-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MAGEB1 antibody (12400-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • IP experiment of HepG2 using 12400-1-AP

    IP experiment of HepG2 using 12400-1-AP

    IP result of anti-MAGEB1 (IP:12400-1-AP, 4ug; Detection:12400-1-AP 1:2000) with HepG2 cells lysate 3360 ug.

MAGEB1 Polyclonal antibody

MAGEB1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 12400-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, IP, ELISA

别名

MAGE Xp, MAGE B1 antigen, DSS-AHC critical interval MAGE superfamily 10, DAM10, CT3.1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, A375 cells
Positive IP detected inHepG2 cells
Positive IF/ICC detected inHepG2 cells, A375 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12400-1-AP targets MAGEB1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag3094

Product name: Recombinant human MAGEB1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-347 aa of BC013772

Sequence: MPRGQKSKLRAREKRRKAREETHYLKVAHATAAEKEECPSSSPVLGDTPTSSPAAGIPQKPQGAPPTTTAAAAVSCTESDEGAKCQGEENASFSQATTSTESSVKDPVAWEAGMLMHFILRKYKMREPIMKADMLKVVDEKYKEYFTEILNGASRRLELVFGLDLKEDNPSGHTYTLVSKLNLTNDGNLSNDWDFPRNGLLMPLLGVIFLKGNSATEEEIWKFMNVLGAYDGEEHLIYGEPRKFITQDLVQEKYLKYEQVPNSDPPRYQFLWGPRAYAETTKMKVLEFLAKMNGATPRDFPSHYEEALRDEEERAQVRSSVRARRRTTATTFRARSRAPFSRSSHPM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 melanoma antigen family B, 1
别名 MAGE Xp, MAGE B1 antigen, DSS-AHC critical interval MAGE superfamily 10, DAM10, CT3.1
计算分子量 347 aa, 39 kDa
观测分子量 43-45 kDa
GenBank蛋白编号BC013772
基因名称 MAGEB1
Gene ID (NCBI) 4112
RRIDAB_2877854
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP43366
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAGEB1, also named as MAGEL1, MAGEXP, DAM10 and CT3.1, is a member of the MAGEB gene family. MAGE genes encode for tumor-rejection antigens and are expressed in tumors of different histologic types and Testis/placenta, but not in other normal tissues. The MW of MAGEB1 is about 40-45kd.

实验方案

Product Specific Protocols
IF protocol for MAGEB1 antibody 12400-1-APDownload protocol
IP protocol for MAGEB1 antibody 12400-1-APDownload protocol
WB protocol for MAGEB1 antibody 12400-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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