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验证数据展示

  • WB analysis using 84248-6-RR (same clone as 84248-6-PBS)

    WB analysis using 84248-6-RR (same clone as 84248-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84248-6-RR (MAGEA4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84248-6-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 84248-6-RR (same clone as 84248-6-PBS)

    FC experiment of A431 using 84248-6-RR (same clone as 84248-6-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug MAGEA4 Recombinant antibody (84248-6-RR, Clone:241558B10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84248-6-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 84248-6-RR (same clone as 84248-6-PBS)

    IF Staining of A431 using 84248-6-RR (same clone as 84248-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using MAGEA4 antibody (84248-6-RR, Clone: 241558B10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84248-6-PBS in a different storage buffer formulation.

MAGEA4 Recombinant monoclonal antibody, PBS Only

MAGEA4 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84248-6-PBS

产品说明书

CloneNo. 241558B10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), Indirect ELISA

别名

241558B10, Cancer/testis antigen 1.4, CT1.4, MAGE 4 antigen, MAGE 41

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84248-6-PBS targets MAGEA4 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag3193

Product name: Recombinant human MAGEA4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-317 aa of BC017723

Sequence: MSSEQKSQHCKPEEGVEAQEEALGLVGAQAPTTEEQEAAVSSSSPLVPGTLEEVPAAESAGPPQSPQGASALPTTISFTCWRQPNEGSSSQEEEGPSTSPDAESLFREALSNKVDELAHFLLRKYRAKELVTKAEMLERVIKNYKRCFPVIFGKASESLKMIFGIDVKEVDPTSNTYTLVTCLGLSYDGLLGNNQIFPKTGLLIIVLGTIAMEGDSASEEEIWEELGVMGVYDGREHTVYGEPRKLLTQDWVQENYLEYRQVPGSNPARYEFLWGPRALAETSYVKVLEHVVRVNARVRIAYPSLREAALLEEEEGV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 melanoma antigen family A, 4
别名 241558B10, Cancer/testis antigen 1.4, CT1.4, MAGE 4 antigen, MAGE 41
计算分子量 317 aa, 35 kDa
观测分子量 45 kDa
GenBank蛋白编号BC017723
基因名称 MAGEA4
Gene ID (NCBI) 4103
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP43358
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MAGEA4 is a member of the MAGEA family, which belongs to chromosome X-clustered cancer/testis antigens. MAGEA4 is expressed in various human cancers but not in normal tissues besides the testis. High expression of MAGE-A4 in tumors was correlated with poor prognosis in many tumor types.

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