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验证数据展示

  • WB analysis using 12508-1-AP

    WB analysis using 12508-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12508-1-AP (MAGEA4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 12508-1-AP

    IHC staining of human lung cancer using 12508-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12508-1-AP (MAGEA4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 12508-1-AP

    IHC staining of human lung cancer using 12508-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12508-1-AP (MAGEA4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of A431 using 12508-1-AP

    FC experiment of A431 using 12508-1-AP

    1x10^6 A431 cells were intracellularly stained with 0.4 ug MAGEA4 Polyclonal antibody (12508-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IHC staining of human breast cancer using 12508-1-AP

    IHC staining of human breast cancer using 12508-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 12508-1-AP (MAGEA4 antibody) at dilution of 1:50 (under 10x lens).

MAGEA4 Polyclonal antibody

MAGEA4 Polyclonal Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 12508-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, FC (Intra), ELISA

别名

MAGE X2, MAGE 41 antigen, MAGE 41, MAGE 4 antigen, CT1.4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA431 cells, U2OS cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inA431 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

12508-1-AP targets MAGEA4 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
文献引用应用IHC
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag3193

Product name: Recombinant human MAGEA4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-317 aa of BC017723

Sequence: MSSEQKSQHCKPEEGVEAQEEALGLVGAQAPTTEEQEAAVSSSSPLVPGTLEEVPAAESAGPPQSPQGASALPTTISFTCWRQPNEGSSSQEEEGPSTSPDAESLFREALSNKVDELAHFLLRKYRAKELVTKAEMLERVIKNYKRCFPVIFGKASESLKMIFGIDVKEVDPTSNTYTLVTCLGLSYDGLLGNNQIFPKTGLLIIVLGTIAMEGDSASEEEIWEELGVMGVYDGREHTVYGEPRKLLTQDWVQENYLEYRQVPGSNPARYEFLWGPRALAETSYVKVLEHVVRVNARVRIAYPSLREAALLEEEEGV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 melanoma antigen family A, 4
别名 MAGE X2, MAGE 41 antigen, MAGE 41, MAGE 4 antigen, CT1.4
计算分子量 317 aa, 35 kDa
观测分子量 45 kDa
GenBank蛋白编号BC017723
基因名称 MAGEA4
Gene ID (NCBI) 4103
RRIDAB_2877863
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP43358
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAGEA4, also named MAGE4 and CT1.4, is one of the germ cell-specific markers (PMID:16540528). SMAGEA4 is one of the subgroup I MAGEAs members which may play important roles in the immune surveillance of certain tumor types and play important roles during embryogenesis.(PMID:15222021) The antibody is specific to MAGEA4.

实验方案

Product Specific Protocols
WB protocol for MAGEA4 antibody 12508-1-APDownload protocol
IHC protocol for MAGEA4 antibody 12508-1-APDownload protocol
FC protocol for MAGEA4 antibody 12508-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Front Endocrinol (Lausanne)

Four novel mutations identification in 17 beta-hydroxysteroid dehydrogenase-3 deficiency and our clinical experience: possible benefits of early treatment

Authors - Yunpeng Wang
View Article