验证数据展示
经过测试的应用
| Positive WB detected in | PC-3 cells, J774A.1 cells, RAW 264.7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11619-1-AP targets MAF1 in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag2198 Product name: Recombinant human MAF1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-256 aa of BC018714 Sequence: MKLLENSSFEAINSQLTVETGDAHIIGRIESYSCKMAGDDKHMFKQFCQEGQPHVLEALSPPQTSGLSPSRLSKSQGGEEEGPLSDKCSRKTLFYLIATLNESFRPDYDFSTARSHEFSREPSLSWVVNAVNCSLFSAVREDFKDLKPQLWNAVDEEICLAECDIYSYNPDLDSDPFGEDGSLWSFNYFFYNKRLKRIVFFSCRSISGSTYTPSEAGNELDMELGEEEVEEESRSRGSGAEETSTMEEDRVPVICI 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | MAF1 homolog (S. cerevisiae) |
| 别名 | MAF 1 |
| 计算分子量 | 256 aa, 29 kDa |
| 观测分子量 | 35 kDa |
| GenBank蛋白编号 | BC018714 |
| 基因名称 | MAF1 |
| Gene ID (NCBI) | 84232 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H063 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MAF1 operates as a conserved repressor of RNA polymerase III transcription, first identified in yeast. It binds RNA pol III directly, blocking TFIIIB recruitment and dampening tRNA and 5S rRNA output. In mammals, MAF1 additionally fine-tunes select RNA pol II genes, impacting metabolism, bone formation, adipogenesis, and oncogenesis, while its own activity is stringently controlled by mTORC1 phosphorylation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MAF1 antibody 11619-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
