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Host
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Species Reactivity
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验证数据展示

  • WB analysis using 83267-2-RR (same clone as 83267-2-PBS)

    WB analysis using 83267-2-RR (same clone as 83267-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83267-2-RR (MAEA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83267-2-PBS in a different storage buffer formulation.

  • FC experiment of U-251 using 83267-2-RR (same clone as 83267-2-PBS)

    FC experiment of U-251 using 83267-2-RR (same clone as 83267-2-PBS)

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug MAEA Recombinant Antibody (83267-2-RR, Clone:240097C9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83267-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83267-2-PBS

    Affinity and Kinetic Characterization of 83267-2-PBS

    Biolayer interferometry (BLl) kinetic assay of 83267-2-PBS against Human MAEA was performed. The affinity constant is below 1 pM.

  • Affinity and Kinetic Characterization of 83267-2-RR

    Affinity and Kinetic Characterization of 83267-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83267-2-RR against Human MAEA were performed. The affinity constant is below 1 pM.

MAEA Recombinant antibody, PBS Only

MAEA Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83267-2-PBS

产品说明书

CloneNo. 240097C9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), Cytometric bead array, Indirect ELISA

别名

240097C9, Cell proliferation-inducing gene 5 protein, E3 ubiquitin-protein transferase MAEA, EC:2.3.2.27, EMLP

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy MAEA Ready-To-Use IHC Kit. MAEA primary antibody included.

产品信息

83267-2-PBS targets MAEA in WB, FC (Intra), Cytometric bead array, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 MAEA fusion protein Ag27150 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 macrophage erythroblast attacher
别名 240097C9, Cell proliferation-inducing gene 5 protein, E3 ubiquitin-protein transferase MAEA, EC:2.3.2.27, EMLP
计算分子量 45 kDa
观测分子量45 kDa
GenBank蛋白编号BC001225
基因名称 MAEA
Gene ID (NCBI) 10296
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ7L5Y9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MAEA(macrophage erythroblast attacher) is a core component of the CTLH E3 ubiquitin-protein ligase complex. MAEA is required for normal cell proliferation and plays a role in erythroblast enucleation during erythrocyte maturation and in the development of mature macrophages. N-terminus MAEA and full-length MAEA are mostly expressed in nuclear and can be detected at low level in the cytoplasm of some cells, while C-terminus MAEA can be detected in the cytoplasm and nucleoli within the nucleus. MAEA mediates the attachment of erythroid cell to mature macrophages which can inhibit erythroid cell apoptosis. The expression of MAEA may be associated with the type 2 diabetes mellitus.(PubMed: 28955747, 24143168, 29911972, 29911972, 30674470, 9763581).