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验证数据展示

  • WB analysis using 83267-1-RR (same clone as 83267-1-PBS)

    WB analysis using 83267-1-RR (same clone as 83267-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83267-1-RR (MAEA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83267-1-PBS in a different storage buffer formulation.

  • FC experiment of U-251 using 83267-1-RR (same clone as 83267-1-PBS)

    FC experiment of U-251 using 83267-1-RR (same clone as 83267-1-PBS)

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug MAEA Recombinant antibody (83267-1-RR, Clone:240097B2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83267-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83267-1-RR

    Affinity and Kinetic Characterization of 83267-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83267-1-RR against Human MAEA were performed. The affinity constant is 0.496 nM.

MAEA Recombinant monoclonal antibody, PBS Only

MAEA Uni-rAb® Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83267-1-PBS

产品说明书

CloneNo. 240097B2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), Cytometric bead array, Indirect ELISA

别名

240097B2, Cell proliferation-inducing gene 5 protein, E3 ubiquitin-protein transferase MAEA, EC:2.3.2.27, EMLP

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy MAEA Ready-To-Use IHC Kit. MAEA primary antibody included.

产品信息

83267-1-PBS targets MAEA in WB, FC (Intra), Cytometric bead array, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag27150

Product name: Recombinant human MAEA protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 26-239 aa of BC001225

Sequence: YETLNKRFRAAQKNIDRETSHVTMVVAELEKTLSGCPAVDSVVSLLDGVVEKLSVLKRKAVESIQAEDESAKLCKRRIEHLKEHSSDQPAAASVWKRKRMDRMMVEHLLRCGYYNTAVKLARQSGIEDLVNIEMFLTAKEVEESLERRETATCLAWCHDNKSRLRKMKSCLEFSLRIQEFIELIRQNKRLDAVRHARKHFSQAEGSQLDEVRQA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
亲和力
KD<1.00 x 10-12M
KOff<1.00 x 10-6M
KOn=9.49 x 104M
全称 macrophage erythroblast attacher
别名 240097B2, Cell proliferation-inducing gene 5 protein, E3 ubiquitin-protein transferase MAEA, EC:2.3.2.27, EMLP
计算分子量 45 kDa
GenBank蛋白编号BC001225
基因名称 MAEA
Gene ID (NCBI) 10296
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ7L5Y9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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