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验证数据展示

  • WB analysis using 15344-1-AP

    WB analysis using 15344-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15344-1-AP (MAD2L1BP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 15344-1-AP

    WB analysis using 15344-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15344-1-AP (MAD2L1BP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin using 15344-1-AP

    IHC staining of human skin using 15344-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 15344-1-AP (MAD2L1BP antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with MAD2L1BP antibody included
  • IHC staining of human skin using 15344-1-AP

    IHC staining of human skin using 15344-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 15344-1-AP (MAD2L1BP antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with MAD2L1BP antibody included
  • IF Staining of A431 using 15344-1-AP

    IF Staining of A431 using 15344-1-AP

    Immunofluorescent analysis of A431 cells using 15344-1-AP (MAD2L1BP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

MAD2L1BP Polyclonal antibody

MAD2L1BP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15344-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

p31(comet), MAD2L1-binding protein, KIAA0110, CMT2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, K-562 cells, HeLa cells, A549 cells, HepG2 cells
Positive IHC detected inhuman skin tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy MAD2L1BP Ready-To-Use IHC Kit. MAD2L1BP primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15344-1-AP targets MAD2L1BP in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 MAD2L1BP fusion protein Ag7550 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 MAD2L1 binding protein
别名 p31(comet), MAD2L1-binding protein, KIAA0110, CMT2
计算分子量 34 kDa
观测分子量 34 kDa
GenBank蛋白编号BC002904
基因名称 MAD2L1BP
Gene ID (NCBI) 9587
RRIDAB_10836940
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15013
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAD2L1BP, also named as CMT2 and KIAA0110, belongs to the MAD2L1BP family. It may function to silence the spindle checkpoint and allow mitosis to proceed through anaphase by binding MAD2L1 after it has become dissociated from the MAD2L1-CDC20 complex. MAD2L1BP interacts with the spindle checkpoint and coordinates cell cycle events in late mitosis. MAD2L1BP may play an active role to remove MAD2 from p55CDC and tether MAD2 for silencing the checkpoint.

实验方案

Product Specific Protocols
WB protocol for MAD2L1BP antibody 15344-1-APDownload protocol
IHC protocol for MAD2L1BP antibody 15344-1-APDownload protocol
IF protocol for MAD2L1BP antibody 15344-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,CoIP

Nat Chem

Multi-tiered chemical proteomic maps of tryptoline acrylamide-protein interactions in cancer cells

Authors - Evert Njomen
  • KO Validated
View Article