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验证数据展示

  • WB analysis using 84398-3-RR

    WB analysis using 84398-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84398-3-RR (MACROD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84398-3-RR

    Affinity and Kinetic Characterization of 84398-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84398-3-RR against Human MACROD1 were performed. The affinity constant is 11.1 nM.

MACROD1 Recombinant antibody

MACROD1 Recombinant Antibody for WB, ELISA
Cat No. 84398-3-RR

产品说明书

CloneNo. 241506C1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

EC:3.2.2.-, EC:3.1.1.106, ADP-ribose glycohydrolase MACROD1, 241506C1, [Protein ADP-ribosylglutamate] hydrolase MACROD1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCOLO 320 cells, HEK-293 cells, HeLa cells, fetal human brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84398-3-RR targets MACROD1 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 MACROD1 fusion protein Ag34080 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 MACRO domain containing 1
别名 EC:3.2.2.-, EC:3.1.1.106, ADP-ribose glycohydrolase MACROD1, 241506C1, [Protein ADP-ribosylglutamate] hydrolase MACROD1
计算分子量 325 aa, 36 kDa
观测分子量 28 kDa
GenBank蛋白编号BC008316
基因名称 MACROD1
Gene ID (NCBI) 28992
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9BQ69
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MACRO domain containing 1 (Macrod1) is also named as LRP16. Macrod1 is an ADP-ribosylhydrolase 1 that is highly enriched in mitochondria, participating in the pathogenesis of cardiovascular diseases (PMID: 38459256). MacroD1 was recruited to the sites of DNA damage via recognition of ADP-ribosylation at DNA lesions (PMID: 32683309). MacroD1 is highly expressed in human and mouse skeletal muscle (PMID: 29410655). Macrod1 may play an important role in carcinogenesis and/or progression of hormone-dependent cancers by feed-forward mechanism that activates ESR1 transactivation (PMID: 17893710, PMID: 17914104). Macrod1 could be involved in invasive growth by down-regulating CDH1 in endometrial cancer cells (PMID: 17893710).

实验方案

Product Specific Protocols
WB protocol for MACROD1 antibody 84398-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols