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验证数据展示

  • WB analysis of Recombinant protein using 85629-1-RR (same clone as 85629-1-PBS)

    WB analysis of Recombinant protein using 85629-1-RR (same clone as 85629-1-PBS)

    Recombinant protein were subjected to SDS PAGE followed by western blot with 85629-1-RR (Lumican antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85629-1-PBS in a different storage buffer formulation.

  • WB analysis using 85629-1-RR (same clone as 85629-1-PBS)

    WB analysis using 85629-1-RR (same clone as 85629-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85629-1-RR (Lumican antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85629-1-PBS in a different storage buffer formulation.

  • WB analysis using 85629-1-RR (same clone as 85629-1-PBS)

    WB analysis using 85629-1-RR (same clone as 85629-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85629-1-RR (Lumican antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85629-1-PBS in a different storage buffer formulation.

  • IHC staining of rat colon using 85629-1-RR (same clone as 85629-1-PBS)

    IHC staining of rat colon using 85629-1-RR (same clone as 85629-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 85629-1-RR (Lumican antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85629-1-PBS in a different storage buffer formulation.

  • IHC staining of rat colon using 85629-1-RR (same clone as 85629-1-PBS)

    IHC staining of rat colon using 85629-1-RR (same clone as 85629-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 85629-1-RR (Lumican antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85629-1-PBS in a different storage buffer formulation.

Rat Lumican Recombinant monoclonal antibody, PBS Only

Lumican Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 85629-1-PBS

产品说明书

CloneNo. 242298G6

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, Indirect ELISA

别名

Keratan sulfate proteoglycan lumican, KSPG lumican, Lcn, Ldc, Lum

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85629-1-PBS targets Lumican in WB, IHC, Indirect ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 mouse, rat
免疫原

CatNo: Eg3410

Product name: Recombinant Rat Lumican protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-N-rFc

Tag: N-rFc

Domain: 19-338 aa of NM_031050.1

Sequence: QYYDYDAPLFMYGELSPNCAPECNCPHSYPTAMYCDDLKLKSVPMVPPGIKYLYLRNNQIDHIDEKAFENVTDLQWLILDHNLLENSKIKGKVFSKLKQLKKLHINYNNLTESVGPLPKSLQDLQLANNKISKLGSFDGLVNLTFIYLQHNQLKEEAVSASLKGLKSLEYLDLSFNQMSKLPAGLPTSLLTLYLDNNKITNIPDEYFNRFTGLQYLRLSHNELADSGVPGNSFNISSLLELDLSYNKLKSIPTVNENLENYYLEVNKLEKFDVKSFCKILGPLSYSKIKHLRLDGNPLTQSSLPPDMYECLRVANEITVN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 lumican
别名 Keratan sulfate proteoglycan lumican, KSPG lumican, Lcn, Ldc, Lum
计算分子量38 kDa
观测分子量50-90 kDa
GenBank蛋白编号NM_031050.1
基因名称 Lum
Gene ID (NCBI) 81682
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP51886
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

umican (LUM), a member of the small leucine-rich proteoglycan (SLRP) family, is one of the major extracellular components in interstitial collagenous matrices of the corneal stroma, aorta, skin, skeletal muscle, lung, kidney, bone, cartilage, and intervertebral discs. SLRPs constitute an important fraction of noncollagenous extracellular matrix proteins and have important effects on cell behavior. Lumican regulates collagenous matrix assembly as a keratan sulfate proteoglycan in the cornea and may exist as a glycoprotein in the connective tissues of other organs. Posttranslational modifications lumican goes through can increase its apparent molecular weight to 50-90 kDa. Studies show that lumican participates in the maintenance of tissue homeostasis and modulates cellular functions including cell proliferation, migration, and differentiation. (PMID: 18949819; 22252641; 9761754; 22365843)

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