验证数据展示
经过测试的应用
| Positive WB detected in | Transfected HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 8 publications below |
| IHC | See 1 publications below |
产品信息
27986-1-AP targets Luciferase in WB, IHC, ELISA applications and shows reactivity with firefly, recombinant protein samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | firefly, recombinant protein |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag27633 Product name: Recombinant photinus pyralis Luciferin 4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 352-550 aa of Sequence: MTPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKSKL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Luciferase |
| 别名 | Luciferin 4-monooxygenase |
| 基因名称 | |
| Gene ID (NCBI) | |
| RRID | AB_2881031 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P08659 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Luciferase produces green light with the wavelength of 562 nm. Luciferase catalyzes the reaction: D-firefly luciferin + O2 + ATP = firefly oxyluciferin + CO2 + AMP + diphosphate + light. This antibody detects the Luciferase protein of Photinus pyralis (Common eastern firefly) (Lampyris pyralis).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Luciferase antibody 27986-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Leucyl-tRNA synthetase is a tumour suppressor in breast cancer and regulates codon-dependent translation dynamics. | ||
EMBO J A stress-induced tyrosine-tRNA depletion response mediates codon-based translational repression and growth suppression. | ||
Nat Commun A plant RNA virus inhibits NPR1 sumoylation and subverts NPR1-mediated plant immunity | ||
J Virol SARS-CoV-2 nsp13 Restricts Episomal DNA Transcription without Affecting Chromosomal DNA | ||
Acta Pharmacol Sin Aiphanol, a multi-targeting stilbenolignan, potently suppresses mouse lymphangiogenesis and lymphatic metastasis |