Livin Polyclonal antibody

Livin Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27543-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human and More (1)

应用

WB, IHC, ELISA

BIRC7, KIAP, LIVIN, ML IAP, MLIAP, RING finger protein 50, RNF50

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inJurkat cells, A431 cells, A375 cells, HeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27543-1-AP targets Livin in WB, IHC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 Human
文献引用反应性human
免疫原 Livin fusion protein Ag26737 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 baculoviral IAP repeat-containing 7
别名 BIRC7, KIAP, LIVIN, ML IAP, MLIAP, RING finger protein 50, RNF50
计算分子量 33 kDa
观测分子量 33 kDa
GenBank蛋白编号BC014475
基因名称 Livin
Gene ID (NCBI) 79444
RRIDAB_2880901
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96CA5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BIRC7 also known as Livin, is one of the family members of the inhibitor of apoptosis protein (IAP). BIRC7 contains a BIR domain that has been shown to bind and inhibit caspases-3, −7 and −9, playing a key role in inhibiting apoptosis. Overexpression of BIRC7 in tumors has been linked with increased chemo- and radio-resistance, recurrence, and decreased patient survival. BIRC7 is closely associated with melanoma disease progression, apoptosis resistance, and chemotherapeutic drug resistance.

实验方案

Product Specific Protocols
WB protocol for Livin antibody 27543-1-APDownload protocol
IHC protocol for Livin antibody 27543-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Int J Oncol

Effects of three-dimensional collagen scaffolds on the expression profiles and biological functions of glioma cells.

Authors - Wei Jia
humanIHC

BMC Cancer

The clinicopathological and prognostic significances of IGF-1R and Livin expression in patients with colorectal cancer.

Authors - Zhenling Zhang
humanWB

Exp Physiol

Oncostatin M upregulates Livin to promote keratinocyte proliferation and survival via ERK and STAT3 signalling pathways.

Authors - Hao Wang
humanWB

Bioengineered

Down-regulation of lncRNA DNAJC3-AS1 inhibits colon cancer via regulating miR-214-3p/LIVIN axis.

Authors - Bing Han
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