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验证数据展示

  • IHC staining of human placenta using 30675-1-AP

    IHC staining of human placenta using 30675-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 30675-1-AP (Lipoprotein(a) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 30675-1-AP

    IF Staining of HepG2 using 30675-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Lipoprotein(a) antibody (30675-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Lipoprotein(a) Polyclonal antibody

Lipoprotein(a) Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 30675-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, ELISA

别名

LPA, Lp(a), LP, Apolipoprotein(a), APOA

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30675-1-AP targets Lipoprotein(a) in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Lipoprotein(a) fusion protein Ag32907 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 lipoprotein, Lp(a)
别名 LPA, Lp(a), LP, Apolipoprotein(a), APOA
计算分子量501 kDa
观测分子量501 kDa
GenBank蛋白编号NM_005577
基因名称 LPA
Gene ID (NCBI) 4018
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP08519
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Lipoprotein(a), also known as Lp(a). The plasma concentration of human lipoprotein(a) is correlated with the risk of heart disease. A distinct feature of the Lp(a) particle is the apolipoprotein (a), which is associated with apoB-100, the main protein component of low-density lipoprotein. It is reported that apo(a), which has extensive homology to plasminogen, binds to immobilized fibronectin (PMID: 2531657). The binding of Lp(a) was localized to the C-terminal heparin-binding domain of fibronectin. It is enriched in liver tissue. The N-glycans are complex biantennary structures present in either a mono- or disialylated state.

实验方案

Product Specific Protocols
IHC protocol for Lipoprotein(a) antibody 30675-1-APDownload protocol
IF protocol for Lipoprotein(a) antibody 30675-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols