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验证数据展示

  • WB analysis using 83853-2-RR

    WB analysis using 83853-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83853-2-RR (Lipocalin-2/NGAL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse spleen using 83853-2-RR

    IHC staining of mouse spleen using 83853-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83853-2-RR (Lipocalin-2/Ngal antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse spleen using 83853-2-RR

    IHC staining of mouse spleen using 83853-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83853-2-RR (Lipocalin-2/Ngal antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83853-2-RR

    Affinity and Kinetic Characterization of 83853-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83853-2-RR against Rat Lipocalin-2/Ngal were performed. The affinity constant is 0.195 nM.

Lipocalin-2/NGAL Recombinant antibody

Lipocalin-2/NGAL Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 83853-2-RR

产品说明书

CloneNo. 240792C12

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, ELISA

别名

Lcn2, NGAL, Lipocalin-2, lipocalin 2, 240792C12

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected in3T3-L1 cells, mouse testis tissue, rat lung tissue
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83853-2-RR targets Lipocalin-2/NGAL in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 mouse, rat
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 lipocalin 2
别名 Lcn2, NGAL, Lipocalin-2, lipocalin 2, 240792C12
计算分子量22kDa
观测分子量22-25 kDa
基因名称 Lipocalin-2/NGAL
Gene ID (NCBI) 170496
RRIDAB_3671438
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP30152
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Neutrophil gelatinase-associated lipocalin (NGAL, also known as lipocalin-2, siderocalin, or LCN2) is a small molecule of almost 25 kDa that belongs to the well-defined superfamily of proteins called lipocalins. NGAL was initially found in activated neutrophils, under its role as an innate antibacterial factor. However, it was subsequently shown that many other types of cells, including those in the kidney tubule, may produce NGAL in response to various injuries. NGAL may become one of the most promising next-generation biomarkers in clinical nephrology and beyond. This antibody recognizes rat Lipocalin-2/Ngal.

实验方案

Product Specific Protocols
WB protocol for Lipocalin-2/NGAL antibody 83853-2-RRDownload protocol
IHC protocol for Lipocalin-2/NGAL antibody 83853-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols