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验证数据展示

  • WB analysis using 82841-1-RR (same clone as 82841-1-PBS)

    WB analysis using 82841-1-RR (same clone as 82841-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82841-1-RR (Lipocalin-2/NGAL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82841-1-PBS in a different storage buffer formulation.

  • WB analysis of HT-29 using 82841-1-RR (same clone as 82841-1-PBS)

    WB analysis of HT-29 using 82841-1-RR (same clone as 82841-1-PBS)

    WB result of NGAL antibody (82841-1-RR; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-NGAL transfected HT-29 cells. This data was developed using the same antibody clone with 82841-1-PBS in a different storage buffer formulation.

  • IHC staining of human intrahepatic cholangiocarcinoma using 82841-1-RR (same clone as 82841-1-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 82841-1-RR (same clone as 82841-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82841-1-RR (NGAL antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82841-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NGAL/LCN2 antibody included
  • Affinity and Kinetic Characterization of 82841-1-RR

    Affinity and Kinetic Characterization of 82841-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82841-1-RR against Human NGAL were performed. The affinity constant is 0.776 nM.

  • Featured Product
  • KD/KO验证

Lipocalin-2/NGAL Recombinant monoclonal antibody, PBS Only

Lipocalin-2/NGAL Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 82841-1-PBS

产品说明书

CloneNo. 2G1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

LCN2, lipocalin 2, NGAL, Lcn 2, HNL

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy NGAL/LCN2 Ready-To-Use IHC Kit. NGAL/LCN2 primary antibody included.

产品信息

82841-1-PBS targets Lipocalin-2/NGAL in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

Recombinant protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 lipocalin 2
别名 LCN2, lipocalin 2, NGAL, Lcn 2, HNL
计算分子量23kd
观测分子量22-25 kDa
GenBank蛋白编号NP_005555.2
基因名称 NGAL/LCN2
Gene ID (NCBI) 3934
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP80188
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Lipocalin-2 (LCN-2) is a adipocytokine also referred to as neutrophil gelatinase-associated lipocalin (NGAL). Lipocalin-2 is a circulatory protein responsible for the transportation of small and hydrophobic molecules to target organs. Lipocalin-2 is used as a biomarker for acute and chronic renal injury. It is present in a large variety of cells including neutrophil, hepatocytes, lung, bone marrow, adipose tissue, macrophages, thymus, non-neoplastic breast duct, prostate, and renal cells. Different functions have been associated with Lipocalin-2, including antibacterial, anti-inflammatory, and protection against cell and tissue stress (PMID:34463264).

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