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验证数据展示

  • WB analysis using 31452-1-AP

    WB analysis using 31452-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31452-1-AP (Lipocalin-2/NGAL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat spleen using 31452-1-AP

    IHC staining of rat spleen using 31452-1-AP

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 31452-1-AP (Lipocalin-2/NGAL antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat spleen using 31452-1-AP

    IHC staining of rat spleen using 31452-1-AP

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 31452-1-AP (Lipocalin-2/NGAL antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Lipocalin-2/NGAL Polyclonal antibody

Lipocalin-2/NGAL Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31452-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

rat

应用

WB, IHC, ELISA

别名

Lcn2, NGAL, Lipocalin-2, lipocalin 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inrat lung tissue, rat spleen tissue, rat testis tissue
Positive IHC detected inrat spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31452-1-AP targets Lipocalin-2/NGAL in WB, IHC, ELISA applications and shows reactivity with rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 rat
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 lipocalin 2
别名 Lcn2, NGAL, Lipocalin-2, lipocalin 2
计算分子量22kDa
观测分子量 25 kDa
基因名称 Lipocalin-2/NGAL
Gene ID (NCBI) 170496
RRIDAB_3669991
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP30152
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Neutrophil gelatinase-associated lipocalin (NGAL, also known as lipocalin-2, siderocalin, or LCN2) is a small molecule of almost 25 kDa that belongs to the well-defined superfamily of proteins called lipocalins. NGAL was initially found in activated neutrophils, under its role as an innate antibacterial factor. However, it subsequently was shown that many other types of cells, including in the kidney tubule, may produce NGAL in response to various injuries. NGAL may become one of the most promising next-generation biomarkers in clinical nephrology and beyond. This antibody recognizes rat Lipocalin-2/NGAL.

实验方案

Product Specific Protocols
WB protocol for Lipocalin-2/NGAL antibody 31452-1-APDownload protocol
IHC protocol for Lipocalin-2/NGAL antibody 31452-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols