验证数据展示

WB analysis using 15677-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15677-1-AP (LZTS2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15677-1-AP
WB result of LZTS2 antibody (15677-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LZTS2 transfected HeLa cells.
IHC staining of human prostate hyperplasia using 15677-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 15677-1-AP (LZTS2 Antibody) at dilution of 1:50 (under 10x lens).
Ready-to-use IHC kits available with LZTS2 antibody included
IHC staining of human prostate hyperplasia using 15677-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 15677-1-AP (LZTS2 Antibody) at dilution of 1:50 (under 40x lens).
Ready-to-use IHC kits available with LZTS2 antibody included
IHC staining of human placenta using 15677-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15677-1-AP (LZTS2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with LZTS2 antibody included
IHC staining of human rectal cancer using 15677-1-AP
Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 15677-1-AP (LZTS2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with LZTS2 antibody included
IHC staining of mouse colon using 15677-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 15677-1-AP (LZTS2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with LZTS2 antibody included
IHC staining of rat stomach using 15677-1-AP
Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 15677-1-AP (LZTS2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with LZTS2 antibody included
IHC staining of mouse stomach using 15677-1-AP
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 15677-1-AP (LZTS2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with LZTS2 antibody included

Featured Product
KD/KO Validated

LZTS2 Polyclonal antibody

LZTS2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15677-1-AP

产品说明书

引用文献(11)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA and More (3)

别名

hLZTS2, LAPSER1, LZTS 2

缓冲液配方: PBS, Azide, Glycerol

PBS, Azide, Glycerol

偶联物: Unconjugated

Unconjugated

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (11)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	HeLa cells, PC-3 cells
Positive IHC detected in	human prostate hyperplasia tissue, human placenta tissue, human rectal cancer tissue, mouse colon tissue, mouse pancreas tissue, mouse stomach tissue, human kidney tissue, rat kidney tissue, rat stomach tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Planning an IHC experiment? We recommend our IHCeasy LZTS2 Ready-To-Use IHC Kit. LZTS2 primary antibody included.

应用	推荐稀释比
Western Blot (WB)	WB : 1:1000-1:8000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 5 publications below
WB	See 9 publications below
IF	See 3 publications below
IP	See 4 publications below
CoIP	See 2 publications below

产品信息

15677-1-AP targets LZTS2 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, ELISA Application Description
文献引用应用	WB, IF, IP, CoIP
经测试反应性	human, mouse, rat
文献引用反应性	human
免疫原	CatNo: Ag8145 Product name: Recombinant human LZTS2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 319-669 aa of BC006212 Sequence: PSDEALLHCVLEGKLRDREAELQQLRDSLDENEATMCQAYEERQRHWQREREALREDCAAQAQRAQRAQQLLQLQVFQLQQEKRQLQDDFAQLLQEREQLERRCATLEREQRELGPRLEETKWEVCQKSGEISLLKQQLKESQAELVQKGSELVALRVALREARATLRVSEGRARGLQEAARARELELEACSQELQRHRQEAEQLREKAGQLDAEAAGLREPPVPPATADPFLLAESDEAKVQRAAAGVGGSLRAQVERLRVELQRERRRGEEQRDSFEGERLAWQAEKEQVIRYQKQLQHNYIQMYRRNRQLEQELQQLSLELEARELADLGLAEQAPCICLEEITATEI 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	leucine zipper, putative tumor suppressor 2
别名	hLZTS2, LAPSER1, LZTS 2
计算分子量	73 kDa
观测分子量	70-80 kDa
GenBank蛋白编号	BC006212
基因名称	LZTS2
Gene ID (NCBI)	84445
RRID	AB_2878166
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	Q9BRK4
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

LZTS2, also named as KIAA1813 and LAPSER1, is a novel b-Catenin-interacting protein and it regulates the nuclear export of b-Catenin. LZTS2 is a negative regulator of katanin-mediated microtubule severing and release from the centrosome. It is required for central spindle formation and the completion of cytokinesis.

实验方案

Product Specific Protocols
IHC protocol for LZTS2 antibody 15677-1-AP	Download protocol
WB protocol for LZTS2 antibody 15677-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
	IF	Nat Commun Proximity proteomics identifies PAK4 as a component of Afadin-Nectin junctions. Authors - Yohendran Baskaran View Article
human	WB	Cell Death Dis RRP15 deficiency induces ribosome stress to inhibit colorectal cancer proliferation and metastasis via LZTS2-mediated β-catenin suppression Authors - Zhixiong Dong View Article
human	WB,IP,CoIP	Cell Death Dis SPOP and HAUSP bidirectionally regulate LZTS2 ubiquitination to modulate the Wnt pathway. Authors - Yanran Deng KD Validated View Article
human	WB	Cancer Lett LZTS2 inhibits PI3K/AKT activation and radioresistance in nasopharyngeal carcinoma by interacting with p85. Authors - Shuangbing Xu KD Validated View Article
human	WB,IP	Oncogene β-Trcp and CK1δ-mediated degradation of LZTS2 activates PI3K/AKT signaling to drive tumorigenesis and metastasis in hepatocellular carcinoma. Authors - Yanwei Lu KD Validated View Article
human	WB	Biochem Pharmacol EBV-encoded miRNAs BHRF1-1 and BART2-5p aggravate post- transplant lymphoproliferative disorder via LZTS2-PI3K-AKT axis Authors - Hao Ji View Article

验证数据展示

WB analysis using 15677-1-AP

WB analysis of HeLa using 15677-1-AP

IHC staining of human prostate hyperplasia using 15677-1-AP

IHC staining of human prostate hyperplasia using 15677-1-AP

IHC staining of human placenta using 15677-1-AP

IHC staining of human rectal cancer using 15677-1-AP

IHC staining of mouse colon using 15677-1-AP

IHC staining of rat stomach using 15677-1-AP

IHC staining of mouse stomach using 15677-1-AP

LZTS2 Polyclonal antibody

LZTS2 Polyclonal Antibody for WB, IHC, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Proximity proteomics identifies PAK4 as a component of Afadin-Nectin junctions.

RRP15 deficiency induces ribosome stress to inhibit colorectal cancer proliferation and metastasis via LZTS2-mediated β-catenin suppression

SPOP and HAUSP bidirectionally regulate LZTS2 ubiquitination to modulate the Wnt pathway.

LZTS2 inhibits PI3K/AKT activation and radioresistance in nasopharyngeal carcinoma by interacting with p85.

β-Trcp and CK1δ-mediated degradation of LZTS2 activates PI3K/AKT signaling to drive tumorigenesis and metastasis in hepatocellular carcinoma.

EBV-encoded miRNAs BHRF1-1 and BART2-5p aggravate post- transplant lymphoproliferative disorder via LZTS2-PI3K-AKT axis

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